Citation
Abdull Rashih A., . and Luqman Chuah Abdullah, . and Fadzureena J., . and Vimala S., . and Pin K. Y., . and Rasadah M. A., . and Mazura M. P., . Antioxidant and anti-inflammatory activities of extracts of betel leaves (Piper betle) from solvents with different polarities. pp. 448-455. ISSN 0128-1283
Abstract
The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water ethanol ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the chemical profiles and concentrations of the active compounds namely hydroxychavicol (HC) and eugenol (EU). The antioxidant potential of the extracts was evaluated using two in vitro assays”xanthine/xanthine oxidase superoxide scavenging assay (SOD assay) and 1 2-diphenyl2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH assay). The anti-inflammatory assays used were hyaluronidase (HYA) xanthine oxidase (XOD) and lipoxygenase (LOX) inhibition assays. The HPLC results revealed that HC and EU were detected in all types of extracts and the concentrations were highest in the water extract. The highest extraction yield was obtained using water. All the extracts were highly active in both antioxidant assays with water extract showing the strongest inhibition. The extracts also exhibited significant inhibition in XOD and LOX assays. The results indicated that the bioactivity of the extracts was related to HC and EU.
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Abstract
The influence of solvents with different polarities on the antioxidant and anti-inflammatory properties of betel leaf extracts (Piper betle) was investigated. The solvents used were water ethanol ethyl acetate and hexane. High performance liquid chromatography (HPLC) was used to determine the chemical profiles and concentrations of the active compounds namely hydroxychavicol (HC) and eugenol (EU). The antioxidant potential of the extracts was evaluated using two in vitro assays”xanthine/xanthine oxidase superoxide scavenging assay (SOD assay) and 1 2-diphenyl2-picrylhydrazyl (DPPH) free radical scavenging assay (DPPH assay). The anti-inflammatory assays used were hyaluronidase (HYA) xanthine oxidase (XOD) and lipoxygenase (LOX) inhibition assays. The HPLC results revealed that HC and EU were detected in all types of extracts and the concentrations were highest in the water extract. The highest extraction yield was obtained using water. All the extracts were highly active in both antioxidant assays with water extract showing the strongest inhibition. The extracts also exhibited significant inhibition in XOD and LOX assays. The results indicated that the bioactivity of the extracts was related to HC and EU.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Antioxidants |
| AGROVOC Term: | Antiinflammatory agents |
| AGROVOC Term: | Piper betle |
| AGROVOC Term: | Solvents |
| AGROVOC Term: | Water |
| AGROVOC Term: | Ethanol |
| AGROVOC Term: | High performance liquid chromatography |
| AGROVOC Term: | Chemical analysis (methods) |
| AGROVOC Term: | Eugenol |
| AGROVOC Term: | Xanthines |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 26 Apr 2025 15:19 |
| URI: | http://webagris.upm.edu.my/id/eprint/21653 |
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