Effect of lipase hydrolysis on the antibacterial activity of coconut oil palm mesocarp oil and selected seed oils against several pathogenic bacteria

Lee S. T., . and Ariffin A., . and Son R., . and Ghazali H. M., . Effect of lipase hydrolysis on the antibacterial activity of coconut oil palm mesocarp oil and selected seed oils against several pathogenic bacteria. pp. 46-54. ISSN 22317546

The antibacterial activity of solvent-extracted oil of noni (Morinda citrifolia L.) spinach (Spinacia oleracea L.) ladys finger (Abelmoschus esculentus (L.) Moench) bitter gourd (Momordica charantia Linn.) and mustard (Brassica nigra L.) seed oils and coconut (Cocos nucifera L.) oil palm (Elaeis guineensis L.) mesocarp in hydrolyzed and unhydrolyzed form were determined in order to explore their potential usage as antibacterial agent. The hydrolysis process that was catalyzed by immobilized lipase of Rhizomucor miehei (RMIM) showed highest hydrolytic activity with 1.0 ml of added water volume except bitter gourd seed oil and palm mesocarp oil which has maximum hydrolytic activity with added water volume of 5 ml and 2.5 ml respectively. Before hydrolysis all oil samples did not show inhibition ring zones (IRZ) on any of the tested bacteria strains (Salmonella typhimurium Listeria monocytogenes and Escherichia coli O157:H7). Hydrolyzed ladys finger and bitter gourd seed oil showed IRZ on all tested bacteria strains; hydrolyzed mustard seed oil on S. typhimurium and L. monocytogenes; hydrolyzed spinach seed oil and coconut oil on L. monocytogenes; hydrolyzed noni seed oil and palm mesocarp oil did not exhibit IRZ on any of the tested bacteria strains. Most of the hydrolyzed oil exhibit an inhibition activity that was different from their respective dominant fatty acids except noni seed oil and palm mesocarp oil.

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(01)%202015/(7).pdf

Abstract

The antibacterial activity of solvent-extracted oil of noni (Morinda citrifolia L.) spinach (Spinacia oleracea L.) ladys finger (Abelmoschus esculentus (L.) Moench) bitter gourd (Momordica charantia Linn.) and mustard (Brassica nigra L.) seed oils and coconut (Cocos nucifera L.) oil palm (Elaeis guineensis L.) mesocarp in hydrolyzed and unhydrolyzed form were determined in order to explore their potential usage as antibacterial agent. The hydrolysis process that was catalyzed by immobilized lipase of Rhizomucor miehei (RMIM) showed highest hydrolytic activity with 1.0 ml of added water volume except bitter gourd seed oil and palm mesocarp oil which has maximum hydrolytic activity with added water volume of 5 ml and 2.5 ml respectively. Before hydrolysis all oil samples did not show inhibition ring zones (IRZ) on any of the tested bacteria strains (Salmonella typhimurium Listeria monocytogenes and Escherichia coli O157:H7). Hydrolyzed ladys finger and bitter gourd seed oil showed IRZ on all tested bacteria strains; hydrolyzed mustard seed oil on S. typhimurium and L. monocytogenes; hydrolyzed spinach seed oil and coconut oil on L. monocytogenes; hydrolyzed noni seed oil and palm mesocarp oil did not exhibit IRZ on any of the tested bacteria strains. Most of the hydrolyzed oil exhibit an inhibition activity that was different from their respective dominant fatty acids except noni seed oil and palm mesocarp oil.

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Item Type:	Article
AGROVOC Term:	Triacylglycerol lipase
AGROVOC Term:	Antibacterial properties
AGROVOC Term:	Fatty acids
AGROVOC Term:	Inhibition
AGROVOC Term:	Pathogenic bacteria
AGROVOC Term:	Coconut oil
AGROVOC Term:	Palm oils
AGROVOC Term:	Morinda citrifolia
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 16:09
URI:	http://webagris.upm.edu.my/id/eprint/21717

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