Extraction and characterisation of -galactosidase produced by Bifidobacterium animalis spp. lactis Bb12 and Lactobacillus delbrueckii spp. bulgaricus ATCC 11842 grown in whey

Prasad L. N, . and Ghosh B. C, . and Sherkat F., . and Shah N. P., . Extraction and characterisation of -galactosidase produced by Bifidobacterium animalis spp. lactis Bb12 and Lactobacillus delbrueckii spp. bulgaricus ATCC 11842 grown in whey. pp. 487-494. ISSN 22317546

This study investigated the production of -Galactosidase (-gal) by Bifidobacterium animalis ssp. lactis Bb12 and Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842 in whey and the effect of four different extraction methods i.e. sonication acetone-toluene SDS-chloroform and lysozyme-EDTA treatment on enzyme activity from these organisms. Both organisms were grown in deproteinised whey containing yeast extract (3.0 g/L) peptone (5.0 g/L) and glucose (10.0 g/L) for 18 h at 37 C for B. animalis ssp. lactis Bb12 and at 45C for L. delbrueckii ssp. bulgaricus ATCC 11842. The optimum intracelluar -gal activity on 15 mM o-nitrophenyl -D-galactopyranoside (ONPG) assay was at pH 6.8 for both organisms irrespective of the method of extraction used. Also the effect of temperature on enzyme activity was studied at various temperatures (30 35 40 45 and 50C). At 35C and 40C B. animalis ssp. lactis Bb12 exhibited more intracellular -gal activity extracted by sonication than other temperatures and methods. However L. delbrueckii ssp. bulgaricus ATCC 11842 showed more intracellular -gal activity at 35C and 45C when extracted by lysozyme-EDTA treatment. Among the four methods used for -gal extraction sonication gave the best result (6.80 Unit/mL) for B. animalis ssp. lactis Bb12 while lysozyme-EDTA treatment was found to be the best (7.77 Unit/mL) for L. delbrueckii ssp. bulgaricus ATCC 11842.

Full text available from: Official URL: http://ifrj.upm.edu.my/20%20(01)%202013/66%20IFRJ%...

Abstract

This study investigated the production of -Galactosidase (-gal) by Bifidobacterium animalis ssp. lactis Bb12 and Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842 in whey and the effect of four different extraction methods i.e. sonication acetone-toluene SDS-chloroform and lysozyme-EDTA treatment on enzyme activity from these organisms. Both organisms were grown in deproteinised whey containing yeast extract (3.0 g/L) peptone (5.0 g/L) and glucose (10.0 g/L) for 18 h at 37 C for B. animalis ssp. lactis Bb12 and at 45C for L. delbrueckii ssp. bulgaricus ATCC 11842. The optimum intracelluar -gal activity on 15 mM o-nitrophenyl -D-galactopyranoside (ONPG) assay was at pH 6.8 for both organisms irrespective of the method of extraction used. Also the effect of temperature on enzyme activity was studied at various temperatures (30 35 40 45 and 50C). At 35C and 40C B. animalis ssp. lactis Bb12 exhibited more intracellular -gal activity extracted by sonication than other temperatures and methods. However L. delbrueckii ssp. bulgaricus ATCC 11842 showed more intracellular -gal activity at 35C and 45C when extracted by lysozyme-EDTA treatment. Among the four methods used for -gal extraction sonication gave the best result (6.80 Unit/mL) for B. animalis ssp. lactis Bb12 while lysozyme-EDTA treatment was found to be the best (7.77 Unit/mL) for L. delbrueckii ssp. bulgaricus ATCC 11842.

[error in script]

Item Type:	Article
AGROVOC Term:	Whey
AGROVOC Term:	Beta galactosidase
AGROVOC Term:	Lysozyme
AGROVOC Term:	Toluene
AGROVOC Term:	Acetone
AGROVOC Term:	Chloroform
AGROVOC Term:	Bifidobacterium
AGROVOC Term:	Lactobacillus
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21754

Actions (login required)

View Item