Occurrence and molecular characterization of some zoonotic bacteria in bovine milk milking equipments and humans in dairy farms Sharkia Egypt

El-Gedawy A. A, . and Ahmed H. A, . and Awadallah M. A. I., . Occurrence and molecular characterization of some zoonotic bacteria in bovine milk milking equipments and humans in dairy farms Sharkia Egypt. pp. 1813-1823. ISSN 22317546

One hundred bulk tank milk samples 50 milking equipments swabs and 50 dairy workers hand swabs were collected from three dairy farms at Sharkia Province Egypt to determine the occurrence of some zoonotic bacteria as well as to investigate the presence of some virulence genes in all isolates by PCR. Staph. aureus St. agalactiae Salmonella species and M. bovis were isolated from milk samples with the percentages of 17 0.04 9 and 1 respectively. In dairy workers hand swabs the respective isolation rates of Staph. aureus St. agalactia and Salmonellae were 10 2 and 8 while only Staph. aureus and St. agalactiae were recovered from milking equipments (6 each). Of the examined Staph. aureus isolates 11.8 and 20 of milk and hand swabs isolates respectively were carrying sea gene. In St. agalactiae isolates cfb virulence gene was identified in all the obtained isolates. Moreover spvC virulence gene was detected in 40 and 33.3 of S. Typhimurium isolates recovered from milk and hand swabs samples respectively. While the respective percentages of spvC gene identification in S. Enteritidis isolates from the same sources were 75 and 100. M. bovis was isolated from 1 of milk samples only. In conclusion the isolation of potentially virulent Staph. aureus St. agalactia and Salmonellae in dairy farms is of public health significance to milk consumers. Although the low isolation rate the detection of M. bovis in milk samples should be of great concern due to its high public health hazards.

Full text available from: Official URL: http://ifrj.upm.edu.my/21%20(05)%202014/15%20IFRJ%...

Abstract

One hundred bulk tank milk samples 50 milking equipments swabs and 50 dairy workers hand swabs were collected from three dairy farms at Sharkia Province Egypt to determine the occurrence of some zoonotic bacteria as well as to investigate the presence of some virulence genes in all isolates by PCR. Staph. aureus St. agalactiae Salmonella species and M. bovis were isolated from milk samples with the percentages of 17 0.04 9 and 1 respectively. In dairy workers hand swabs the respective isolation rates of Staph. aureus St. agalactia and Salmonellae were 10 2 and 8 while only Staph. aureus and St. agalactiae were recovered from milking equipments (6 each). Of the examined Staph. aureus isolates 11.8 and 20 of milk and hand swabs isolates respectively were carrying sea gene. In St. agalactiae isolates cfb virulence gene was identified in all the obtained isolates. Moreover spvC virulence gene was detected in 40 and 33.3 of S. Typhimurium isolates recovered from milk and hand swabs samples respectively. While the respective percentages of spvC gene identification in S. Enteritidis isolates from the same sources were 75 and 100. M. bovis was isolated from 1 of milk samples only. In conclusion the isolation of potentially virulent Staph. aureus St. agalactia and Salmonellae in dairy farms is of public health significance to milk consumers. Although the low isolation rate the detection of M. bovis in milk samples should be of great concern due to its high public health hazards.

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Item Type:	Article
AGROVOC Term:	Dairy farms
AGROVOC Term:	Staphylococcus aureus
AGROVOC Term:	Streptococcus agalactiae
AGROVOC Term:	Salmonella
AGROVOC Term:	Mycobacterium bovis
AGROVOC Term:	Virulence
AGROVOC Term:	Milk
AGROVOC Term:	Egypt
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21853

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