Evaluation of in vitro immunomodulatory effect of fractions of Ficus septica Burm. f. and their total flavonoid and phenolic contents


Citation

Kunti Nastiti, . and Sudarsono, . and Agung Endro Nugroho, . Evaluation of in vitro immunomodulatory effect of fractions of Ficus septica Burm. f. and their total flavonoid and phenolic contents. pp. 1981-1987. ISSN 22317546

Abstract

The study aimed to evaluate the in vitro effects of some fractions of ethanolic extract of F. septica Burm. f. on Balb/c mice macrophage phagocytosis and lymphocyte proliferation. In addition we also determined the total flavonoid and phenolic contents in the fractions. These fractions were n-hexane soluble fraction ethyl acetate soluble fraction and ethyl acetate insoluble fraction. These fractions were then tested on lymphocytes proliferation by a MTT reduction method and on macrophage phagocytosis activity based on the number of latex beads uptake by the cells. In the study the total phenolics content of ethyl acetate-soluble ethyl acetate-insoluble and n-hexane soluble fractions were 35.53; 7.59 and 0.33 respectively. Whereas their total flavonoids content were 3.27; 0.81 and 0.05 respectively. All fractions exhibited to increase the lymphocytes proliferation and macrophage phagocytosis activity. Among them ethyl acetate fraction had highest immunomodulatory effects. In conclusion ethyl acetate fraction of ethanolic extract of Ficus septica Burm. f is potential to develop as an immunomodulatory agent.


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Abstract

The study aimed to evaluate the in vitro effects of some fractions of ethanolic extract of F. septica Burm. f. on Balb/c mice macrophage phagocytosis and lymphocyte proliferation. In addition we also determined the total flavonoid and phenolic contents in the fractions. These fractions were n-hexane soluble fraction ethyl acetate soluble fraction and ethyl acetate insoluble fraction. These fractions were then tested on lymphocytes proliferation by a MTT reduction method and on macrophage phagocytosis activity based on the number of latex beads uptake by the cells. In the study the total phenolics content of ethyl acetate-soluble ethyl acetate-insoluble and n-hexane soluble fractions were 35.53; 7.59 and 0.33 respectively. Whereas their total flavonoids content were 3.27; 0.81 and 0.05 respectively. All fractions exhibited to increase the lymphocytes proliferation and macrophage phagocytosis activity. Among them ethyl acetate fraction had highest immunomodulatory effects. In conclusion ethyl acetate fraction of ethanolic extract of Ficus septica Burm. f is potential to develop as an immunomodulatory agent.

Additional Metadata

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Item Type: Article
AGROVOC Term: Ficus
AGROVOC Term: Stimulant immunomodulators
AGROVOC Term: In vitro
AGROVOC Term: Flavonoids
AGROVOC Term: Phenolic content
AGROVOC Term: Macrophages
AGROVOC Term: Phagocytosis
AGROVOC Term: Lymphocyte proliferation
AGROVOC Term: Latex
AGROVOC Term: Cancer (disease)
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/21880

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