Market surveillance on non-halal additives incorporated in surimi based products using polymerase chain reaction (PCR)“southern hybridization analysis

Aravindran S., . and Sahilah A. M., . and Aminah A., . Market surveillance on non-halal additives incorporated in surimi based products using polymerase chain reaction (PCR)“southern hybridization analysis. pp. 2095-2099. ISSN 22317546

In Malaysia halal certification status for some surimi-based product is still suspicious due to the incorporation of non-halal plasma protein additives as part of the food ingredient. This study was conducted to determine the presence of plasma protein additives that have been incorporated into surimi-based product using Polymerase Chain Reaction (PCR)-Southern Hybridization method which able to differentiate 7 type (beef chicken duck goat buffalo lamb and pork) of species on a single chip. A random of 17 (n 173) different brands of surimi-based product was purchased from Selangor local market in January 2013. Of 17 brands 3 (n 33) brands were positive for chicken DNA and 1 (n 13) brand was positive for goat DNA while remainder 13 brands (n 133) has no DNA species detected. In presence study it is evidence that PCR-Southern Hybridization analysis offered a reliable result due to its highly specific and sensitive properties in detecting plasma protein incorporation in surimi-based product.

Full text available from: Official URL: http://ifrj.upm.edu.my/21%20(06)%202014/5%20IFRJ%2...

Abstract

In Malaysia halal certification status for some surimi-based product is still suspicious due to the incorporation of non-halal plasma protein additives as part of the food ingredient. This study was conducted to determine the presence of plasma protein additives that have been incorporated into surimi-based product using Polymerase Chain Reaction (PCR)-Southern Hybridization method which able to differentiate 7 type (beef chicken duck goat buffalo lamb and pork) of species on a single chip. A random of 17 (n 173) different brands of surimi-based product was purchased from Selangor local market in January 2013. Of 17 brands 3 (n 33) brands were positive for chicken DNA and 1 (n 13) brand was positive for goat DNA while remainder 13 brands (n 133) has no DNA species detected. In presence study it is evidence that PCR-Southern Hybridization analysis offered a reliable result due to its highly specific and sensitive properties in detecting plasma protein incorporation in surimi-based product.

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Item Type:	Article
AGROVOC Term:	Surimi
AGROVOC Term:	Food additives
AGROVOC Term:	Plasma proteins
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	DNA hybridization
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 15:54
URI:	http://webagris.upm.edu.my/id/eprint/21908

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