Molecular characterization of Escherichia coli isolated from different food sources

Son R., . and Tay L. W., . and Nakaguchi T., . and Cheah Y. K., . and Aida A. A., . and Nishibuchi M., . Molecular characterization of Escherichia coli isolated from different food sources. pp. 31-40. ISSN 22317546

Escherichia coli and Escherichia coli O157 were identified from selom� (Oenanthe stolonifera) pegaga� (Centella asiatica) beef chicken lamb buffalo ulam Raja� (Cosmos caudatus) and tenggek burung� (Euodia redlevi). The bacteria were recovered using chromagenic agar. Isolated Escherichia coli and Escherichia coli 0157 were further characterized by plasmid profiling and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The virulence genes of the isolates (VT1 VT2 LT ST eaeA inV) that produces pathogenic Escherichia coli and 16S rRNA gene were screened by a multiplex PCR assay. The plasmid profiling analysis showed that out of 176 isolates only 103 isolates contained plasmids. ERIC-PCR analysis generated amplified products in the range of 150 bp to 1000 bp categorizing isolates into a total of 52 different profiles. Multiplex PCR showed that 20 (32.3) of the isolates carried eaeA gene 6 (9.7) isolates possessed inV genes only 1 (1.6) have VT2 genes and 1 (1.6) as well carried VT1 genes 2 (3.2) of the isolates harboured LT genes and only 1 (1.6) isolate possessed ST genes. There were no correlation between plasmid ERIC-PCR and virulence genes profiles.

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(01)%202015/(5).pdf

Abstract

Escherichia coli and Escherichia coli O157 were identified from selom� (Oenanthe stolonifera) pegaga� (Centella asiatica) beef chicken lamb buffalo ulam Raja� (Cosmos caudatus) and tenggek burung� (Euodia redlevi). The bacteria were recovered using chromagenic agar. Isolated Escherichia coli and Escherichia coli 0157 were further characterized by plasmid profiling and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The virulence genes of the isolates (VT1 VT2 LT ST eaeA inV) that produces pathogenic Escherichia coli and 16S rRNA gene were screened by a multiplex PCR assay. The plasmid profiling analysis showed that out of 176 isolates only 103 isolates contained plasmids. ERIC-PCR analysis generated amplified products in the range of 150 bp to 1000 bp categorizing isolates into a total of 52 different profiles. Multiplex PCR showed that 20 (32.3) of the isolates carried eaeA gene 6 (9.7) isolates possessed inV genes only 1 (1.6) have VT2 genes and 1 (1.6) as well carried VT1 genes 2 (3.2) of the isolates harboured LT genes and only 1 (1.6) isolate possessed ST genes. There were no correlation between plasmid ERIC-PCR and virulence genes profiles.

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Item Type:	Article
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Plasmids
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Virulence
AGROVOC Term:	Isolation techniques
AGROVOC Term:	Beef
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 16:09
URI:	http://webagris.upm.edu.my/id/eprint/21966

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