Antioxidant and inhibitory effects of aqueous and ethanolic extract of Tapinanthus bangwensis leaves on Fe2-induced lipid peroxidation in pancreas (in vitro)

Molehin O. R., . and Adefegha S. A., . Antioxidant and inhibitory effects of aqueous and ethanolic extract of Tapinanthus bangwensis leaves on Fe2-induced lipid peroxidation in pancreas (in vitro). pp. 269-274. ISSN 22317546

This study was designed to investigate and compare the aqueous (AQTB) and ethanolic (ETTB) extracts of Tapinanthus bangwensis leaves on the phenolic content antioxidant activity and inhibitory effect on Fe2- induced lipid peroxidation in pancreas (in vitro). Antioxidant properties (Inhibition of Fe2-induced lipid peroxidation in rat pancreas total phenol and total flavonoid contents ferric reducing antioxidant property nitric oxide radical scavenging ability using in vitro model were evaluated. The result revealed that the ETTB had higher total phenol and total flavonoid contents than AQTB. Furthermore ETTB exhibited strong antioxidant properties as observed by its nitric oxide (NO) radical scavenging ability and ferric reducing antioxidant property than AQTB.The result also revealed that incubation of rat pancreas in the presence of 25 M Fe2 caused a significant increase (p 0.05) in malondiadehyde (MDA) contents of the rat pancreas (156.9) when compared with the basal pancreas homogenate (100). However AQTB showed higher inhibitory effects on pancreatic MDA produced in a dose-dependent pattern (0 - 0.63 mg/mL) than the ETTB. The antioxidant activity and inhibitory effect of both extracts against lipid peroxidation could be attributed to the phenolic phytochemicals present in the extracts. It could therefore be concluded that both extracts protect the pancreas against oxidative damage but aqueous extract exhibited the better protection.

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(01)%202015/(38).pdf

Abstract

This study was designed to investigate and compare the aqueous (AQTB) and ethanolic (ETTB) extracts of Tapinanthus bangwensis leaves on the phenolic content antioxidant activity and inhibitory effect on Fe2- induced lipid peroxidation in pancreas (in vitro). Antioxidant properties (Inhibition of Fe2-induced lipid peroxidation in rat pancreas total phenol and total flavonoid contents ferric reducing antioxidant property nitric oxide radical scavenging ability using in vitro model were evaluated. The result revealed that the ETTB had higher total phenol and total flavonoid contents than AQTB. Furthermore ETTB exhibited strong antioxidant properties as observed by its nitric oxide (NO) radical scavenging ability and ferric reducing antioxidant property than AQTB.The result also revealed that incubation of rat pancreas in the presence of 25 M Fe2 caused a significant increase (p 0.05) in malondiadehyde (MDA) contents of the rat pancreas (156.9) when compared with the basal pancreas homogenate (100). However AQTB showed higher inhibitory effects on pancreatic MDA produced in a dose-dependent pattern (0 - 0.63 mg/mL) than the ETTB. The antioxidant activity and inhibitory effect of both extracts against lipid peroxidation could be attributed to the phenolic phytochemicals present in the extracts. It could therefore be concluded that both extracts protect the pancreas against oxidative damage but aqueous extract exhibited the better protection.

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Item Type:	Article
AGROVOC Term:	Ethanol
AGROVOC Term:	Lipid peroxidation
AGROVOC Term:	Pancreas
AGROVOC Term:	Phenolic content
AGROVOC Term:	Antioxidants
AGROVOC Term:	Flavonoids
AGROVOC Term:	Mistletoe
AGROVOC Term:	Antioxidative compounds
AGROVOC Term:	Medicinal herbs
AGROVOC Term:	Medicinal plants
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21996

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