Influence of different 2 4-D concentrations on antioxidant contents and activities in sweet basil leaf-derived callus during proliferation

Wongsen W., . and Bodhipadma K., . and Noichinda S., . and Leung D. W. M., . Influence of different 2 4-D concentrations on antioxidant contents and activities in sweet basil leaf-derived callus during proliferation. pp. 638-643. ISSN 22317546

The potential of sweet basil callus cultures as a source of natural antioxidants was investigated. Sweet basil (Ocimum basilicum L.) leaves were cultured on a semi-solid nutrient medium supplemented with 0 0.5 1 or 2 mg/l 2 4-D under a light illumination regime (20 mol m-2 s-1 for 16 h and 8 h in the dark daily) at 25C for 4 weeks. All the media used except the plant growth regulator-free medium could induce formation of green compact callus. After 4 weeks callus cultured on the medium supplemented with 0.5 mg/l 2 4-D had the highest fresh weight. Antioxidant content analysis showed that this callus culture had the highest levels of -carotene ascorbic acid phenolics and flavonoids (0.08 mg/gFW 0.64 mg/gFW 6.54 mg gallic acid/gFW and 7.38 mg rutin/gFW respectively) after 1 week of callus proliferation. An evaluation of antioxidant ability suggested that changes in reducing power ascorbic acid content DPPH free radical scavenging activity phenolics and flavonoids contents in sweet basil leaf-derived callus might all be interlinked.

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(02)%202015/(27).pdf

Abstract

The potential of sweet basil callus cultures as a source of natural antioxidants was investigated. Sweet basil (Ocimum basilicum L.) leaves were cultured on a semi-solid nutrient medium supplemented with 0 0.5 1 or 2 mg/l 2 4-D under a light illumination regime (20 mol m-2 s-1 for 16 h and 8 h in the dark daily) at 25C for 4 weeks. All the media used except the plant growth regulator-free medium could induce formation of green compact callus. After 4 weeks callus cultured on the medium supplemented with 0.5 mg/l 2 4-D had the highest fresh weight. Antioxidant content analysis showed that this callus culture had the highest levels of -carotene ascorbic acid phenolics and flavonoids (0.08 mg/gFW 0.64 mg/gFW 6.54 mg gallic acid/gFW and 7.38 mg rutin/gFW respectively) after 1 week of callus proliferation. An evaluation of antioxidant ability suggested that changes in reducing power ascorbic acid content DPPH free radical scavenging activity phenolics and flavonoids contents in sweet basil leaf-derived callus might all be interlinked.

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Item Type:	Article
AGROVOC Term:	Auxins
AGROVOC Term:	Basil
AGROVOC Term:	Ocimum basilicum
AGROVOC Term:	Natural antioxidants
AGROVOC Term:	Callus culture
AGROVOC Term:	Flavonoids
AGROVOC Term:	Phenolic content
AGROVOC Term:	Cultivated plants
AGROVOC Term:	Herbs (herbaceous plants)
AGROVOC Term:	Health
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22041

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