Total phenolic content and in vitro antioxidant activity of Vigna sinensis

Azlina M., . and Siti Syakiroh Z.A., . and Noor Husna Z., . and Siti Nafizah M.B., . and Norhayati A.H., . and Ummu Habibah A., . and Nor Farhanah M.Y., . and Ahmad Zubaidi A.L., . and Mohd Adzim Khalili R., . and Emynur Shafekh S., . and Catherine C.C.W., . and Noor Shahhida Sazura A.R., . Total phenolic content and in vitro antioxidant activity of Vigna sinensis. pp. 1393-1400. ISSN 2231-7546

Vigna sinensis also known as long-podded cowpea or Chinese long bean (Family:Fabaceae) is most widely grown in Southeast Asia. They are a good source of protein vitamin A vitamin C iron phosphorus and potassium. The antioxidant potential of crude methanol extract chloroform and ethyl acetate soluble fractions of Vigna sinensiswas screened for in- vitro antioxidant activity using total phenolic content ferric reducing power 2 2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay ferric thiocyanate (FTC) and thiobarbituric acid (TBA) tests. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (2.69 mg/g GAE in concentration 0.5 mg/mL); more effective than methanol and chloroform extract.This fraction also exhibited fairly good antioxidant activity with in both TBA (17.39 mg/g GAE) and FTC (12.65 mg/g GAE) methods.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(04)%202012/14%20I...

Abstract

Vigna sinensis also known as long-podded cowpea or Chinese long bean (Family:Fabaceae) is most widely grown in Southeast Asia. They are a good source of protein vitamin A vitamin C iron phosphorus and potassium. The antioxidant potential of crude methanol extract chloroform and ethyl acetate soluble fractions of Vigna sinensiswas screened for in- vitro antioxidant activity using total phenolic content ferric reducing power 2 2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay ferric thiocyanate (FTC) and thiobarbituric acid (TBA) tests. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (2.69 mg/g GAE in concentration 0.5 mg/mL); more effective than methanol and chloroform extract.This fraction also exhibited fairly good antioxidant activity with in both TBA (17.39 mg/g GAE) and FTC (12.65 mg/g GAE) methods.

[error in script]

Item Type:	Article
AGROVOC Term:	Phenolic content
AGROVOC Term:	Antioxidants
AGROVOC Term:	Vigna sinensis
AGROVOC Term:	Cowpeas
AGROVOC Term:	Fabaceae
AGROVOC Term:	Vitamin c
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22046

Actions (login required)

View Item