Maximising phycocyanin extraction from a newly identified Egyptian cyanobacteria strain: Anabaena oryzae SOS13

Abdel Ghany A., . and Salama A., . and Osman A., . and Sitohy M., . Maximising phycocyanin extraction from a newly identified Egyptian cyanobacteria strain: Anabaena oryzae SOS13. pp. 517-525. ISSN 22317546

A potentially promising Egyptian cyanobacterial isolate (Anabaena oryzae) was identified by morphological biochemical approaches as well as sequencing a fragment of the 16S rDNA gene as new strain denoted as Anabaena oryzae SOS13. The new strain was used to produce phycocyanin through extraction using a physical (freeze-thaw) or an enzymatic (lysozyme) approach. Combined freezing-thawing treatments were associated with different levels of phycocyanin yield where the combination -50C x 25C achieved complete extraction of the three pigments (100 each). The enzymatic extraction of the three pigments was significantly influenced by the temperature giving maximum yields at 40C but was much less effective than the physical freeze-thaw method that is more economic and less liable to potential chemical changes. The resulting extract could be easily purified by the two step ammonium sulfate fractionation giving a product with two SDS-PAGE bands (15 kD and 25 k

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(02)%202015/(11).pdf

Abstract

A potentially promising Egyptian cyanobacterial isolate (Anabaena oryzae) was identified by morphological biochemical approaches as well as sequencing a fragment of the 16S rDNA gene as new strain denoted as Anabaena oryzae SOS13. The new strain was used to produce phycocyanin through extraction using a physical (freeze-thaw) or an enzymatic (lysozyme) approach. Combined freezing-thawing treatments were associated with different levels of phycocyanin yield where the combination -50C x 25C achieved complete extraction of the three pigments (100 each). The enzymatic extraction of the three pigments was significantly influenced by the temperature giving maximum yields at 40C but was much less effective than the physical freeze-thaw method that is more economic and less liable to potential chemical changes. The resulting extract could be easily purified by the two step ammonium sulfate fractionation giving a product with two SDS-PAGE bands (15 kD and 25 k

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Item Type:	Article
AGROVOC Term:	Cyanobacteria
AGROVOC Term:	Anabaena
AGROVOC Term:	Extraction in chemical analysis
AGROVOC Term:	Freeze thaw cycles (soil)
AGROVOC Term:	Lysozyme
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22057

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