Evaluation of total phenolic content total antioxidant activity and antioxidant vitamin composition of pomegranate seed and juice


Citation

Anahita A., . and Asmah R., . and Fauziah O., . Evaluation of total phenolic content total antioxidant activity and antioxidant vitamin composition of pomegranate seed and juice. pp. 1212-1217. ISSN 22317546

Abstract

This study aimed to determine total phenolic content (TPC) total antioxidant activity (TAA) antioxidant vitamin composition (A C and E) of pomegranate fruit. In addition two edible parts of pomegranate juice pomegranate seed and combination of them were compared based on antioxidant properties. TPC was determined by using Folin-Ciocalteu (FC) method based on colorimetric reduction. Ferric reduction ability power (FRAP assay) was used to test the antioxidant activity. Vitamin assessments were conducted by using high performance liquid chromatography (HPLC). Results for antioxidant vitamin composition in pomegranate juice (PJ) showed that the concentration of vitamin A was 22.8 0.69 g/100 g vitamin C was 57.8 0.59 mg/100 g and vitamin E was 0.07 0.01 mg/100 g. Besides TPC in PJ pomegranate seed (PS) and pomegranate seed-juice (PSJ) was 2502 54 165 49 and 2696 49 mg GAE/L and TAA was 32 5.1 20 2.8 and 47 5.5 mmol/L respectively. This study revealed that PSJ contained high level of phenolic compounds antioxidant activity and vitamin C. In addition TPC was as main contributor to antioxidant activities and positively correlated with TAA (r2 0.91 p0.05). Therefore combination of pomegranate seed and juice may become an alternative and potential source of natural antioxidant.


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Abstract

This study aimed to determine total phenolic content (TPC) total antioxidant activity (TAA) antioxidant vitamin composition (A C and E) of pomegranate fruit. In addition two edible parts of pomegranate juice pomegranate seed and combination of them were compared based on antioxidant properties. TPC was determined by using Folin-Ciocalteu (FC) method based on colorimetric reduction. Ferric reduction ability power (FRAP assay) was used to test the antioxidant activity. Vitamin assessments were conducted by using high performance liquid chromatography (HPLC). Results for antioxidant vitamin composition in pomegranate juice (PJ) showed that the concentration of vitamin A was 22.8 0.69 g/100 g vitamin C was 57.8 0.59 mg/100 g and vitamin E was 0.07 0.01 mg/100 g. Besides TPC in PJ pomegranate seed (PS) and pomegranate seed-juice (PSJ) was 2502 54 165 49 and 2696 49 mg GAE/L and TAA was 32 5.1 20 2.8 and 47 5.5 mmol/L respectively. This study revealed that PSJ contained high level of phenolic compounds antioxidant activity and vitamin C. In addition TPC was as main contributor to antioxidant activities and positively correlated with TAA (r2 0.91 p0.05). Therefore combination of pomegranate seed and juice may become an alternative and potential source of natural antioxidant.

Additional Metadata

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Item Type: Article
AGROVOC Term: Phenolic content
AGROVOC Term: Antioxidants
AGROVOC Term: Vitamins
AGROVOC Term: Pomegranates
AGROVOC Term: Punica granatum
AGROVOC Term: Ascorbic acid
AGROVOC Term: Acetic acid
AGROVOC Term: Gallic acid
AGROVOC Term: Epidemiology
AGROVOC Term: Chemicals
Geographical Term: Malaysia
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 26 Apr 2025 16:39
URI: http://webagris.upm.edu.my/id/eprint/22105

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