Biomass production by Yarrowia lipolytica as a source of lipids: bench scale cultivation on raw glycerol-based medium

Dalcanton F., . and Michelon M., . and Furlong E. B., . and Burkert J. F. M., . and Machado Junior F. R. S., . and Burkert C. A. V., . Biomass production by Yarrowia lipolytica as a source of lipids: bench scale cultivation on raw glycerol-based medium. pp. 1253-1260. ISSN 22317546

This paper analyzes the aeration and agitation effects in growth parameters and lipid content for Yarrowia lipolytica NRRL YB-423 growing in a raw glycerol-based medium. A central composite design (22 assays plus three central points) was made with combinations of agitation (100 to 300 rpm) and aeration (0.5 to 1.5 vvm). The resulting KLa values ranged from 2.25 to 16.83 h-1. The most favorable condition was 200 rpm and 1 vvm (KLa of 8.29) producing 19.14 g L-1 biomass with a lipid content of 7.87. In relation to fatty acid profile polyunsaturated fatty acids prevailed (62.55) achieving 49.16 linoleic acid (C18:2). Therefore this yeast biomass has the potential to constitute a sustainable and renewable alternative as a source of essential fatty acids in food formulations for animals and as a raw material for the development of glycerol-based biorefineries for fuels and chemicals.

Full text available from: Official URL: http://ifrj.upm.edu.my/22%20(03)%202015/(52).pdf

Abstract

This paper analyzes the aeration and agitation effects in growth parameters and lipid content for Yarrowia lipolytica NRRL YB-423 growing in a raw glycerol-based medium. A central composite design (22 assays plus three central points) was made with combinations of agitation (100 to 300 rpm) and aeration (0.5 to 1.5 vvm). The resulting KLa values ranged from 2.25 to 16.83 h-1. The most favorable condition was 200 rpm and 1 vvm (KLa of 8.29) producing 19.14 g L-1 biomass with a lipid content of 7.87. In relation to fatty acid profile polyunsaturated fatty acids prevailed (62.55) achieving 49.16 linoleic acid (C18:2). Therefore this yeast biomass has the potential to constitute a sustainable and renewable alternative as a source of essential fatty acids in food formulations for animals and as a raw material for the development of glycerol-based biorefineries for fuels and chemicals.

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Item Type:	Article
AGROVOC Term:	Yarrowia lipolytica
AGROVOC Term:	Lipids
AGROVOC Term:	Biomass
AGROVOC Term:	Cultivation
AGROVOC Term:	Glycerol
AGROVOC Term:	Aeration
AGROVOC Term:	Growth rate
AGROVOC Term:	Lipid content
AGROVOC Term:	Fatty acids
AGROVOC Term:	Linoleic acid
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22111

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