Optimization of -galactosidase production by response surface methodology using locally isolated Kluyveromyces marxianus


Citation

Al- jazairi M., . and Abou-ghorra S., . and Bakri Y., . and Mustafa M., . Optimization of -galactosidase production by response surface methodology using locally isolated Kluyveromyces marxianus. pp. 1361-1367. ISSN 22317546

Abstract

Five parameters including initial sugar concentration agitation speed initial pH incubation time and temperature were studied for the optimization of -galactosidase production in synthetic medium containing lactose as carbon source by Kluyveromyces marxianus DIYS11 and using Response Surface Methodology (RSM) as statistical analysis. The optimum conditions for the highest enzyme activity were sugar concentration 10 agitation speed 250 rpm pH 3 incubation time 64 hours and temperature20C which generated 4997 U/ml/min and made the yeast Kluyveromyces marxianus DIYS11 a promise organism for industrial -galactosidase production and the RSM a good tool for the optimization of enzyme production.


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Abstract

Five parameters including initial sugar concentration agitation speed initial pH incubation time and temperature were studied for the optimization of -galactosidase production in synthetic medium containing lactose as carbon source by Kluyveromyces marxianus DIYS11 and using Response Surface Methodology (RSM) as statistical analysis. The optimum conditions for the highest enzyme activity were sugar concentration 10 agitation speed 250 rpm pH 3 incubation time 64 hours and temperature20C which generated 4997 U/ml/min and made the yeast Kluyveromyces marxianus DIYS11 a promise organism for industrial -galactosidase production and the RSM a good tool for the optimization of enzyme production.

Additional Metadata

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Item Type: Article
AGROVOC Term: Beta galactosidase
AGROVOC Term: Kluyveromyces marxianus
AGROVOC Term: Sugar
AGROVOC Term: Temperature
AGROVOC Term: Lactose
AGROVOC Term: Enzymes
AGROVOC Term: Fermentation
AGROVOC Term: Microorganisms
AGROVOC Term: Enzyme activity
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/22126

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