Citation
Santiago L., . and Bartolome M., . Chromatographic separation of the free radical scavenging components of the leaf extracts of Leea philippinensis Merr. pp. 1396-1403. ISSN 22317546
Abstract
The ability of Leea philippinensis an endemic tree in the Philippines as in vitro free radical scavenger was investigated. Soxhlet extraction using solvents in increasing order of polarity (hexanechloroformethyl acetateacetonemethanol) yielded acetone and methanol extracts with the highest Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). These crude extracts were further separated using silica gel column chromatography with eluents hexane hexane:acetone (1:1) acetone acetone:methanol (1:1) and methanol. Fractions with the most TFC and TPC exhibited concentration-dependent activity with an IC50 value range of 0.10 - 0.29 0.17 - 0.80 0.29 - 0.84 and 3.40 - 9.75 mg/mL respectively against DPPH hydroxyl nitric oxide and hydrogen peroxide inhibition assays. In addition these fractions demonstrated reducing power by transforming the Fe3 to Fe2. Infrared spectra of fractions at 4000“500 cm-1 using FTIR revealed presence of phenolic group.
Download File
Full text available from:
Official URL: http://ifrj.upm.edu.my/22%20(04)%202015/(12).pdf
|
Abstract
The ability of Leea philippinensis an endemic tree in the Philippines as in vitro free radical scavenger was investigated. Soxhlet extraction using solvents in increasing order of polarity (hexanechloroformethyl acetateacetonemethanol) yielded acetone and methanol extracts with the highest Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). These crude extracts were further separated using silica gel column chromatography with eluents hexane hexane:acetone (1:1) acetone acetone:methanol (1:1) and methanol. Fractions with the most TFC and TPC exhibited concentration-dependent activity with an IC50 value range of 0.10 - 0.29 0.17 - 0.80 0.29 - 0.84 and 3.40 - 9.75 mg/mL respectively against DPPH hydroxyl nitric oxide and hydrogen peroxide inhibition assays. In addition these fractions demonstrated reducing power by transforming the Fe3 to Fe2. Infrared spectra of fractions at 4000“500 cm-1 using FTIR revealed presence of phenolic group.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Chromatography |
| AGROVOC Term: | Free radicals |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Polarity |
| AGROVOC Term: | Chloroform |
| AGROVOC Term: | Acetone |
| AGROVOC Term: | Methanol |
| AGROVOC Term: | Phenolic content |
| AGROVOC Term: | Flavonoids |
| AGROVOC Term: | Gel chromatography |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/22131 |
Actions (login required)
 |
View Item |
