Effect of honey supplementation into Tris Extender on Cryopreservation of Bull Spermatozoa

Sarsaifi K., . and Muhammad N., . and Wahid H., . and Khumran A. M., . and Yimer N., . and Kaka A., . and Rosnina Y., . Effect of honey supplementation into Tris Extender on Cryopreservation of Bull Spermatozoa. pp. 47-54. ISSN 1394-3277

The aim of this study was to evaluate the effects of using honey as a supplement into Tris extender in comparison with a commercial Bioxcell extender on bull semen cryopreservation quality. Semen samples from four fertile bulls which comprised of two Friesian Sahiwal one Braford and one Kedah-Kelantan breeds were collected using an electro-ejaculation method. Collected samples were freshly evaluated physically and microscopically before divided and diluted using four Tris-based extenders containing 0 (control) 0.025 5 and 10 honey and a commercial Bioxcell extender. The processed semen was equilibrated at 5C for 3 h (chilling) packed into straws (0.25 ml) frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Semen quality parameters used for evaluation and comparison after chilling and thawing included general and progressive motility (analyzed using a computer assisted semen analyzer) livability and sperm morphology (done using eosin-nigrosin staining technique). The experiment revealed that among all Tris-based extenders improved post-cryopreservation semen quality was obtained from Tris2.5 honey; comparable to Bioxcell which was superior to all other extenders. Use of honey at concentrations of 5 and 10 however were not suitable and showed significantly poor semen quality following cryopreservation. Therefore Tris extender with 2.5 honey could be considered as a cheaper alternative to Bioxcell as it could be prepared in the laboratory whenever needed.

Full text available from: Official URL: http://www.msap.my/pdf/mjas_18_2/5.Kazhar-effect_r...

Abstract

The aim of this study was to evaluate the effects of using honey as a supplement into Tris extender in comparison with a commercial Bioxcell extender on bull semen cryopreservation quality. Semen samples from four fertile bulls which comprised of two Friesian Sahiwal one Braford and one Kedah-Kelantan breeds were collected using an electro-ejaculation method. Collected samples were freshly evaluated physically and microscopically before divided and diluted using four Tris-based extenders containing 0 (control) 0.025 5 and 10 honey and a commercial Bioxcell extender. The processed semen was equilibrated at 5C for 3 h (chilling) packed into straws (0.25 ml) frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Semen quality parameters used for evaluation and comparison after chilling and thawing included general and progressive motility (analyzed using a computer assisted semen analyzer) livability and sperm morphology (done using eosin-nigrosin staining technique). The experiment revealed that among all Tris-based extenders improved post-cryopreservation semen quality was obtained from Tris2.5 honey; comparable to Bioxcell which was superior to all other extenders. Use of honey at concentrations of 5 and 10 however were not suitable and showed significantly poor semen quality following cryopreservation. Therefore Tris extender with 2.5 honey could be considered as a cheaper alternative to Bioxcell as it could be prepared in the laboratory whenever needed.

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Item Type:	Article
AGROVOC Term:	Honey
AGROVOC Term:	Food supplementation
AGROVOC Term:	Cryopreservation
AGROVOC Term:	Spermatozoa
AGROVOC Term:	Bulls
AGROVOC Term:	Semen
AGROVOC Term:	Breeds
AGROVOC Term:	Electroejaculation
AGROVOC Term:	Chilling
AGROVOC Term:	Nitrogen
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 16:45
URI:	http://webagris.upm.edu.my/id/eprint/22162

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