Citation
Natesha S. R., . and Vijayakumar N. K., . In vitro propagation of Ailanthus triphysa. pp. 402-412. ISSN 0128-1283
Abstract
In vitro propagation ofthe tropical tree species Ailanthus tnphysa using axillary and terminal bud explants from 3-to 4-year-old saplings was attempted. Murashige and Skoog basal medium was the best medium for culture establishment and shoot growth. Among the various cytokinins supplemented to the basal medium singly or in combination with indole-3-acetic acid (1M) Nb-benzyladenine (RA) at 3.0 mg 1-1 was betterfor leafand multiple shootproduction. Combinationsoftwocytokinins namely BA (3.0 mg I-I) and kinetin (1.0 mg I-I) produced multiple shoots with highest mean number of shoots (4.3). However shoot elongation was very limited in all growth regulator combinations tested for shoot production. Rooting of microshoots was successfully accomplished in half-strength Murashige and Skoog medium supplemented with both 4.0 mg 1-1 IAA and 0.4 mg I-I indole-3-butyric acid.
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Abstract
In vitro propagation ofthe tropical tree species Ailanthus tnphysa using axillary and terminal bud explants from 3-to 4-year-old saplings was attempted. Murashige and Skoog basal medium was the best medium for culture establishment and shoot growth. Among the various cytokinins supplemented to the basal medium singly or in combination with indole-3-acetic acid (1M) Nb-benzyladenine (RA) at 3.0 mg 1-1 was betterfor leafand multiple shootproduction. Combinationsoftwocytokinins namely BA (3.0 mg I-I) and kinetin (1.0 mg I-I) produced multiple shoots with highest mean number of shoots (4.3). However shoot elongation was very limited in all growth regulator combinations tested for shoot production. Rooting of microshoots was successfully accomplished in half-strength Murashige and Skoog medium supplemented with both 4.0 mg 1-1 IAA and 0.4 mg I-I indole-3-butyric acid.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Ailanthus |
| AGROVOC Term: | In vitro |
| AGROVOC Term: | Plant propagation |
| AGROVOC Term: | Tropical forests |
| AGROVOC Term: | Cytokinins |
| AGROVOC Term: | Kinetin |
| AGROVOC Term: | Cell elongation |
| AGROVOC Term: | Plant growth regulators |
| AGROVOC Term: | Micropropagation |
| AGROVOC Term: | Tissue culture |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/22236 |
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