Induction of Androgenic embryoids from another culture of Madhuca indica

Sarkar D., . and Maity S., . and Das P. K., . Induction of Androgenic embryoids from another culture of Madhuca indica. pp. 429-436. ISSN 0128-1283

The effects of anther development sucrose and growth regulators 011 the embryogenic response of Madhuca indica anthers were studied. Anthers isolated from 8 12 and 18 mOl flower buds were cultured on Murashige and Skoogs medium supplemented with sucrose (30 and 60 g 1-1) and varying concentrations of 2 4-dichlorophenoxyacetic acid (2 4-D) (4.5 and 9.1 Ml in combination with Nl;-benzyladenine (BA) (4.4 and 8.9 M) or kinetin (4.6 and 9.3 J.1M). Difference in response was observed between various developmental stages ofanthers. Anthers taken from 12 mOl flower buds (stage II anthers) were most responsive in terms of survival as well as callogenic ditlerentiation. Stage III anthers (from 18 mm flower buds) were the least responsive in culture. Cytological observations showed that microspores in both stage I (from 8 mm flower buds) and stage II anthers were predominantly uninucleate. Optimum callus proliferation from stage II anthers occurred in low (30 g I-I) sucrose medium supplemented with 2 4-D and BA. Embryoid induction took place exclusively from stage II anthers at a higher sucrose (60 g I-I) concentration in the presence of high levels of 2 4-D and kinetin. Early embryonic segmentatiull in stage II microspores indicated androgenic origin of these embryoids. However these embryoids faikd to regenerate into complete plantlets suggeMing restricted morpho-organogenic competence of anthers in M. indica.

Full text available from: Official URL: http://www.frim.gov.my/v1/JTFSOnline/jtfs/v16n4/42...

Abstract

The effects of anther development sucrose and growth regulators 011 the embryogenic response of Madhuca indica anthers were studied. Anthers isolated from 8 12 and 18 mOl flower buds were cultured on Murashige and Skoogs medium supplemented with sucrose (30 and 60 g 1-1) and varying concentrations of 2 4-dichlorophenoxyacetic acid (2 4-D) (4.5 and 9.1 Ml in combination with Nl;-benzyladenine (BA) (4.4 and 8.9 M) or kinetin (4.6 and 9.3 J.1M). Difference in response was observed between various developmental stages ofanthers. Anthers taken from 12 mOl flower buds (stage II anthers) were most responsive in terms of survival as well as callogenic ditlerentiation. Stage III anthers (from 18 mm flower buds) were the least responsive in culture. Cytological observations showed that microspores in both stage I (from 8 mm flower buds) and stage II anthers were predominantly uninucleate. Optimum callus proliferation from stage II anthers occurred in low (30 g I-I) sucrose medium supplemented with 2 4-D and BA. Embryoid induction took place exclusively from stage II anthers at a higher sucrose (60 g I-I) concentration in the presence of high levels of 2 4-D and kinetin. Early embryonic segmentatiull in stage II microspores indicated androgenic origin of these embryoids. However these embryoids faikd to regenerate into complete plantlets suggeMing restricted morpho-organogenic competence of anthers in M. indica.

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Item Type:	Article
AGROVOC Term:	Madhuca indica
AGROVOC Term:	Anthers
AGROVOC Term:	Sucrose
AGROVOC Term:	Plant growth regulators
AGROVOC Term:	Flowers
AGROVOC Term:	Buds
AGROVOC Term:	Benzyladenine
AGROVOC Term:	Plant biotechnology
AGROVOC Term:	Callus
AGROVOC Term:	Haploidy
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22237

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