High performance amylolytic yeast strains isolation and identification for valorization of potatoes waste available in Burkina Faso

Zongo C., . and Savadogo A., . and Somda K. M., . and Traoré A. S., . and Ouédraogo N., . High performance amylolytic yeast strains isolation and identification for valorization of potatoes waste available in Burkina Faso. pp. 1463-1469. ISSN 2231-7546

This study deals with isolation and identification of high amylolytic activity strains from potato wastes for biomass production. A total of twenty five (25) yeast strains were isolated using standard microbiological methods. Ten (10) strains were retained on the basis of their capacity to hydrolyze starch. Identification to species level of selected strains was performed with an API 20C AUX (Bio-Mrieux) according to the manufacturers instructions. Strain NOY 1 was identified as Candida utilis and had the best diameter of starch hydrolysis (24 mm) and the optimal enzymatic activity (1248 M.l-1.mn-1). Optimal growth parameters of this strain were 30C pH 5; under these conditions 5 g of starch/liter of medium were used for biomass production in an incubator at 150 rpm for 72 hours. The maximal biomass and protein content were obtained after 36 and 40 hours of incubation and were 5.65 g/l of medium and 0.53 g/g of dry weight respectively

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(04)%202012/25%20I...

Abstract

This study deals with isolation and identification of high amylolytic activity strains from potato wastes for biomass production. A total of twenty five (25) yeast strains were isolated using standard microbiological methods. Ten (10) strains were retained on the basis of their capacity to hydrolyze starch. Identification to species level of selected strains was performed with an API 20C AUX (Bio-Mrieux) according to the manufacturers instructions. Strain NOY 1 was identified as Candida utilis and had the best diameter of starch hydrolysis (24 mm) and the optimal enzymatic activity (1248 M.l-1.mn-1). Optimal growth parameters of this strain were 30C pH 5; under these conditions 5 g of starch/liter of medium were used for biomass production in an incubator at 150 rpm for 72 hours. The maximal biomass and protein content were obtained after 36 and 40 hours of incubation and were 5.65 g/l of medium and 0.53 g/g of dry weight respectively

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Item Type:	Article
AGROVOC Term:	Yeasts
AGROVOC Term:	Potatoes
AGROVOC Term:	Wastes
AGROVOC Term:	Candida utilis
AGROVOC Term:	Hydrolysis
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22240

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