The curative action of Lactobacillus plantarum FNCC 226 to Saprolegnia parasitica A3 on catfish (Pangasius hypophthalamus Sauvage)

Nurhajati J., . and Atira, . and Aryantha I. N. P., . and Kadek Indah D. G., . The curative action of Lactobacillus plantarum FNCC 226 to Saprolegnia parasitica A3 on catfish (Pangasius hypophthalamus Sauvage). pp. 1723-1727. ISSN 2231-7546

The inhibition of the growth of the parasitic S. parasitica A3 on catfish using L. plantarum FNCC 226 in vivo and in vitro were examined in this study. To prevent fungal proliferation different concentration of S. parasitica A3 were employed in a two step approach: to determine the concentration of zoospore suspension count that caused a 50 infection on catfish and concentration of L. plantarum FNCC 226 that inhibits the growth of the S. parasitica A3 on catfish. The design use was the Completely Randomized Design with 4 x4 factorial pattern consisting of 4 infection concentration of S. parasitica A3 suspensions i.e. 0xIC50 IC50 (Infectious Concentration 50) 2xIC50 and 4xIC50 and 4 concentrations of L. plantarum FNCC 226 inoculums i.e. MaxNLC (Maximal Nir Lethal Concentration) xMaxNLC xMaxNLC and 0xMaxNLC with 4 times replication. The results obtained showed that the IC50 count for a 50 infectious was 1 x 107 zoospore/mL and were dose dependent. L. plantarum FNCC 226 inhibit mycelium of S. parasitica A3 were found to be 7.7 x 105 cfu/mL (MaxNLC) 4.0 x 105 cfu/mL (xMaxNLC) and 2.3 x 105 cfu/mL (xMaxNLC) and were dependent on the initial number of the infectious S. parasitica A3. The decrement of S. parasitica A3 infection coincide with the increment of L. plantarum FNCC 226 blocked. Thus this study indicated that L. plantarum has the capacity to inhibit S. parasitica and it would be possible to design new biocontrol of this pathogen in catfish.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(04)%202012/58%20I...

Abstract

The inhibition of the growth of the parasitic S. parasitica A3 on catfish using L. plantarum FNCC 226 in vivo and in vitro were examined in this study. To prevent fungal proliferation different concentration of S. parasitica A3 were employed in a two step approach: to determine the concentration of zoospore suspension count that caused a 50 infection on catfish and concentration of L. plantarum FNCC 226 that inhibits the growth of the S. parasitica A3 on catfish. The design use was the Completely Randomized Design with 4 x4 factorial pattern consisting of 4 infection concentration of S. parasitica A3 suspensions i.e. 0xIC50 IC50 (Infectious Concentration 50) 2xIC50 and 4xIC50 and 4 concentrations of L. plantarum FNCC 226 inoculums i.e. MaxNLC (Maximal Nir Lethal Concentration) xMaxNLC xMaxNLC and 0xMaxNLC with 4 times replication. The results obtained showed that the IC50 count for a 50 infectious was 1 x 107 zoospore/mL and were dose dependent. L. plantarum FNCC 226 inhibit mycelium of S. parasitica A3 were found to be 7.7 x 105 cfu/mL (MaxNLC) 4.0 x 105 cfu/mL (xMaxNLC) and 2.3 x 105 cfu/mL (xMaxNLC) and were dependent on the initial number of the infectious S. parasitica A3. The decrement of S. parasitica A3 infection coincide with the increment of L. plantarum FNCC 226 blocked. Thus this study indicated that L. plantarum has the capacity to inhibit S. parasitica and it would be possible to design new biocontrol of this pathogen in catfish.

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Item Type:	Article
AGROVOC Term:	Saprolegnia
AGROVOC Term:	Mycelium
AGROVOC Term:	Freshwater fishes
AGROVOC Term:	Fish
AGROVOC Term:	Lactobacillus plantarum
AGROVOC Term:	Lactic acid
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22271

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