In vitro effect on the antioxidative properties of crude extract of Chenopodium album in presence of the organophosphate acephate


Citation

Sanjukta D., . and Ghosh S., . In vitro effect on the antioxidative properties of crude extract of Chenopodium album in presence of the organophosphate acephate. pp. 1033-1039. ISSN 2231-7546

Abstract

Acephate an organophosphorus pesticide is a potent free radical generator. Phenolic compounds present in leafy vegetables are recently gaining interest as good sources of natural antioxidants. The aim of this study was thus to evaluate the role of plant phenolics present in Chenopodium album as a free radical scavenger which is a commonly consumed green leafy vegetable against acephate toxicity in vitro due to its free radical generation ability. An attempt was also made to identify a few of the phenolic compounds present in the crude extract through RP-HPLC. DPPH radical scavenging activity and reducing power of the leafy vegetable extract both in the presence and absence of acephate were conducted using concentrations of 1g/ml 2.5 g/ml and 5 g/ml of polyphenolic compounds present in the extract and a concentration of 3 mg/ml of the pesticide. The results showed that acephate significantly reduced the radical scavenging activity of the leafy vegetable extract in comparison to the tests containing only the vegetable extract as well as a higher concentration was required to quench the ABTS. radicals in the presence of acephate. No significant change was observed in case of inhibition of lipid peroxidation in a linoleic acid emulsion system in the presence of acephate when compared to the tests containing only the plant extract. Flavonoids like rutin quercetin and rutin hydrate were identified as few of the phenolic components present in the crude C.album extract. Thus from this study it is evident that pesticides act as a serious free radical source whose deleterious effects can be reduced if not totally but to a certain extent by such green leafy vegetables which contain a wide array of natural antioxidants in the form of polyphenols preventing unnecessary oxidation reactions.


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Abstract

Acephate an organophosphorus pesticide is a potent free radical generator. Phenolic compounds present in leafy vegetables are recently gaining interest as good sources of natural antioxidants. The aim of this study was thus to evaluate the role of plant phenolics present in Chenopodium album as a free radical scavenger which is a commonly consumed green leafy vegetable against acephate toxicity in vitro due to its free radical generation ability. An attempt was also made to identify a few of the phenolic compounds present in the crude extract through RP-HPLC. DPPH radical scavenging activity and reducing power of the leafy vegetable extract both in the presence and absence of acephate were conducted using concentrations of 1g/ml 2.5 g/ml and 5 g/ml of polyphenolic compounds present in the extract and a concentration of 3 mg/ml of the pesticide. The results showed that acephate significantly reduced the radical scavenging activity of the leafy vegetable extract in comparison to the tests containing only the vegetable extract as well as a higher concentration was required to quench the ABTS. radicals in the presence of acephate. No significant change was observed in case of inhibition of lipid peroxidation in a linoleic acid emulsion system in the presence of acephate when compared to the tests containing only the plant extract. Flavonoids like rutin quercetin and rutin hydrate were identified as few of the phenolic components present in the crude C.album extract. Thus from this study it is evident that pesticides act as a serious free radical source whose deleterious effects can be reduced if not totally but to a certain extent by such green leafy vegetables which contain a wide array of natural antioxidants in the form of polyphenols preventing unnecessary oxidation reactions.

Additional Metadata

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Item Type: Article
AGROVOC Term: Chenopodium
AGROVOC Term: Phenolic compounds
AGROVOC Term: In vitro
AGROVOC Term: Free radicals
AGROVOC Term: Acephate
AGROVOC Term: Polyphenols
AGROVOC Term: Lipid peroxidation
AGROVOC Term: Crude oil
AGROVOC Term: Extracts
AGROVOC Term: Toxicity
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/22338

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