Simultaneous determination of heavy metals in drinking waters and wines by methods: Ion pair-reversed phase high performance liquid chromatography and capillary zone electrophoresis

Sakai T., . and Santaladchaiyakit Y., . and Srijaranai S., . and Pimrote W., . and Kukusamude C., . Simultaneous determination of heavy metals in drinking waters and wines by methods: Ion pair-reversed phase high performance liquid chromatography and capillary zone electrophoresis. pp. 1079-1088. ISSN 2231-7546

Simultaneous determination of metal ions in drinking waters and wines using ion paired reversed phase high performance liquid chromatography (IPRPHPLC) and capillary zone electrophoresis (CZE) have been investigated. Co(II) Cu(II) Ni(II) Fe(II) and V(V) are analyzed as their Nitro-PAPS chelates. Reversed flow injection (rFI) is used for in-line complexation prior to their separation by IP-RPHPLC. The optimum mobile phase compositions obtained from sequential simplex method by carried out on a C18 Bondapak column at a flow rate of 1.0 mL min-1 and detection at 570 nm are 39.7 acetonitrile containing 5.5 mmol L-1 acetate buffer 5.0 containing 3.5 mmol L-1 tetrabutylammonium bromide (TBABr). Meanwhile CZE condition is performed on an uncoated fused-silica capillary (30.0 cm effective length) with the electrolyte of 35 mmol L-1 borate buffer pH 10.0 in 10 acetonitrile and 0.04 mmol L-1 Nitro-PAPS separation voltage of 15 kV and detection at 250 nm. The analytical performance of both methods are examined and discussed. The analysis time of IP-RPHPLC including in-line complexation by rFI and separation of five metals is 19 min whereas the analysis time of CZE is 7 min. A high risk of iron and copper contamination is found in all drinking water and wine samples studied. The results obtained from both proposed methods are comparable (t-test 95 confidence limit).

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(03)%202012/(40)%2...

Abstract

Simultaneous determination of metal ions in drinking waters and wines using ion paired reversed phase high performance liquid chromatography (IPRPHPLC) and capillary zone electrophoresis (CZE) have been investigated. Co(II) Cu(II) Ni(II) Fe(II) and V(V) are analyzed as their Nitro-PAPS chelates. Reversed flow injection (rFI) is used for in-line complexation prior to their separation by IP-RPHPLC. The optimum mobile phase compositions obtained from sequential simplex method by carried out on a C18 Bondapak column at a flow rate of 1.0 mL min-1 and detection at 570 nm are 39.7 acetonitrile containing 5.5 mmol L-1 acetate buffer 5.0 containing 3.5 mmol L-1 tetrabutylammonium bromide (TBABr). Meanwhile CZE condition is performed on an uncoated fused-silica capillary (30.0 cm effective length) with the electrolyte of 35 mmol L-1 borate buffer pH 10.0 in 10 acetonitrile and 0.04 mmol L-1 Nitro-PAPS separation voltage of 15 kV and detection at 250 nm. The analytical performance of both methods are examined and discussed. The analysis time of IP-RPHPLC including in-line complexation by rFI and separation of five metals is 19 min whereas the analysis time of CZE is 7 min. A high risk of iron and copper contamination is found in all drinking water and wine samples studied. The results obtained from both proposed methods are comparable (t-test 95 confidence limit).

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Item Type:	Article
AGROVOC Term:	Wines
AGROVOC Term:	Drinking water
AGROVOC Term:	Heavy metals
AGROVOC Term:	Electrophoresis
AGROVOC Term:	High performance liquid chromatography
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22343

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