The best method for isolated total RNA from durian tissues

Ky H., . and Yeap S. K., . and Napis S. B., . The best method for isolated total RNA from durian tissues. pp. 1181-1183. ISSN 2231-7546

Plant tissues especially durian tissues contain high content of polysaccharides polyphenols and other secondary metabolites which can co-precipitate with RNA causing problem in further transcriptomic study. In this experiment three basic chaotic agents CTAB SDS and guanidine are used in three basic protocols for RNA isolation. The effectiveness of each method was determined by spectrophotometer denaturing agarose gels analysis and northern blot hybridization. CTAB combining with additional sodium acetate precipitation step showed highest yield and best quality of isolated RNA which was free from contaminations of polysaccharides polyphenols and other secondary metabolites. Furthermore the total RNA from 4-month old durian flesh of clone D24 was successfully used to construct a cDNA library. In conclusion CTAB method is effective to isolate total RNA on various types of durian tissues for further gene expression analysis.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(03)%202012/(53)%2...

Abstract

Plant tissues especially durian tissues contain high content of polysaccharides polyphenols and other secondary metabolites which can co-precipitate with RNA causing problem in further transcriptomic study. In this experiment three basic chaotic agents CTAB SDS and guanidine are used in three basic protocols for RNA isolation. The effectiveness of each method was determined by spectrophotometer denaturing agarose gels analysis and northern blot hybridization. CTAB combining with additional sodium acetate precipitation step showed highest yield and best quality of isolated RNA which was free from contaminations of polysaccharides polyphenols and other secondary metabolites. Furthermore the total RNA from 4-month old durian flesh of clone D24 was successfully used to construct a cDNA library. In conclusion CTAB method is effective to isolate total RNA on various types of durian tissues for further gene expression analysis.

[error in script]

Item Type:	Article
AGROVOC Term:	RNA
AGROVOC Term:	Durian
AGROVOC Term:	Durio zibethinus
AGROVOC Term:	Plant tissues
AGROVOC Term:	Polysaccharides
AGROVOC Term:	Polyphenols
AGROVOC Term:	Guanidines
AGROVOC Term:	Spectrophotometers
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22360

Actions (login required)

View Item