Molecular characterization of clinical isolate of Vibrio cholerae isolated from outbreaks cases in Malaysia

Patrick G. B., . and Nishibuchi M., . and Tunung R., . and Son Radu, . Molecular characterization of clinical isolate of Vibrio cholerae isolated from outbreaks cases in Malaysia. pp. 1267-1274. ISSN 2231-7546

A total of 32 clinical strains of Vibrio cholerae including members of the 01 and 0139 serogroup were collected from Klang Selangor; Penang Island; Samarahan Sarawak and Miri Sarawak in Malaysia. In general all the isolates except the 0139 serotype expressed low resistance to all the antibiotics tested with their Multiple Antibiotic Resistance (MAR) indices ranged from 0.10 to 0.48. The presence of ctx gene that encoded the cholera toxin was confirmed in all these clinical isolates by polymerase chain reaction. The results from the RAPD-PCR were analyzed using the RAPDistance software (Version 1.04). From the dendrogram generated two main groups were observed which were subdivided into two clusters each. The Selangors isolates and the 0139 Penangs isolates formed one group whereas the Samarahan Sarawak isolates and the Miri Sarawak isolates made up the other group thus delineating their different sources of origin based on their geographical location.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(03)%202012/(66)%2...

Abstract

A total of 32 clinical strains of Vibrio cholerae including members of the 01 and 0139 serogroup were collected from Klang Selangor; Penang Island; Samarahan Sarawak and Miri Sarawak in Malaysia. In general all the isolates except the 0139 serotype expressed low resistance to all the antibiotics tested with their Multiple Antibiotic Resistance (MAR) indices ranged from 0.10 to 0.48. The presence of ctx gene that encoded the cholera toxin was confirmed in all these clinical isolates by polymerase chain reaction. The results from the RAPD-PCR were analyzed using the RAPDistance software (Version 1.04). From the dendrogram generated two main groups were observed which were subdivided into two clusters each. The Selangors isolates and the 0139 Penangs isolates formed one group whereas the Samarahan Sarawak isolates and the Miri Sarawak isolates made up the other group thus delineating their different sources of origin based on their geographical location.

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Item Type:	Article
AGROVOC Term:	Vibrio cholerae
AGROVOC Term:	Disease outbreak
AGROVOC Term:	Antibiotic resistance
AGROVOC Term:	PCR
AGROVOC Term:	Cholera
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 16:55
URI:	http://webagris.upm.edu.my/id/eprint/22372

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