Yeast identification from domestic ragi for food fermentation by PCR method

Noorhisham T.K., . and Siti Hajar M.D., . and Nurina A., . Yeast identification from domestic ragi for food fermentation by PCR method. pp. 775-777. ISSN 2231-7546

In this study polymerase chain reaction (PCR) was used to identify yeast in domestic ragi obtained from two local markets in Sarawak and Pahang. These ragi are normally used as a dry starter in food fermentation (tapai) for Pahang (ST2) and Sarawak (ST3) and tuak (ST1) which is an alcoholic drink in Sarawak. Universal primer NL1 and NL4 were used as a primer in this study to amplify D1/D2 fragment. Based on the result from the sequencing and after the BLAST search of the nucleotide sequences the strain was confirmed as Candida glabrata (FN424108.) partial 26S rRNA gene strain IMUFRJ 51955 for ST1 Saccharomyces cerevisiae (EU285514.1) isolate 35 26S ribosomal RNA gene partial sequence for ST2 sample and Candida glabrata (FN393990.1) partial 26S rRNA gene strain MUCL 51244 for ST3. All these strains were found in domestic ragi used for food fermentation.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/19%20(02)%202012/(61)IF...

Abstract

In this study polymerase chain reaction (PCR) was used to identify yeast in domestic ragi obtained from two local markets in Sarawak and Pahang. These ragi are normally used as a dry starter in food fermentation (tapai) for Pahang (ST2) and Sarawak (ST3) and tuak (ST1) which is an alcoholic drink in Sarawak. Universal primer NL1 and NL4 were used as a primer in this study to amplify D1/D2 fragment. Based on the result from the sequencing and after the BLAST search of the nucleotide sequences the strain was confirmed as Candida glabrata (FN424108.) partial 26S rRNA gene strain IMUFRJ 51955 for ST1 Saccharomyces cerevisiae (EU285514.1) isolate 35 26S ribosomal RNA gene partial sequence for ST2 sample and Candida glabrata (FN393990.1) partial 26S rRNA gene strain MUCL 51244 for ST3. All these strains were found in domestic ragi used for food fermentation.

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Item Type:	Article
AGROVOC Term:	Polymerase chain reaction
AGROVOC Term:	Ragi
AGROVOC Term:	Fermented foods
AGROVOC Term:	Alcoholic beverages
AGROVOC Term:	Saccharomyces cerevisiae
AGROVOC Term:	Candida glabrata
AGROVOC Term:	Yeasts
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22395

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