Citation
Son R., . and Vengadesh L., . and Yoke-Kqueen C., . Molecular quantitation and characterization of Vibrio cholerae from different seafood obtained from wetmarket and supermarket. pp. 45-50. ISSN 2231-7546
Abstract
Vibrio cholerae still represents a significant threat to human health worldwide despite the advances in hygiene consumer knowledge food treatment and food processing. In Malaysia statistics in year 2009 have shown that among the food and water borne diseases food poisoning has the highest incidence rate of 36.17 per 100 000 populations and with a mortality rate of 0.01 per 100 000 populations. In this study 22 seafood samples comprising of fish squid crustacean and mollusks purchased from wet market and supermarket were analyzed. The Most Probable Number (MPN) and real time PCR was used to enumerate the Vibrio cholerae in seafood sample. The results showed that MPN-real time PCR of the samples from wet market had a maximum of 1100 MPN/g compare to 93 MPN/g enumerated from the MPN plate. The MPN-real time PCR in the samples from supermarket indicated 290 MPN/g as compared to 240 MPN/g enumerated from the MPN plate. The standard curves showed that there was a good linear correlation between the Ct values. The minimum level of detection of Vibrio cholerae standard DNA at targeted gene was 3 x 10-5 ng/l.
Download File
Full text available from:
Official URL: http://www.ifrj.upm.edu.my/19%20(01)%202011/(6)IFR...
|
Abstract
Vibrio cholerae still represents a significant threat to human health worldwide despite the advances in hygiene consumer knowledge food treatment and food processing. In Malaysia statistics in year 2009 have shown that among the food and water borne diseases food poisoning has the highest incidence rate of 36.17 per 100 000 populations and with a mortality rate of 0.01 per 100 000 populations. In this study 22 seafood samples comprising of fish squid crustacean and mollusks purchased from wet market and supermarket were analyzed. The Most Probable Number (MPN) and real time PCR was used to enumerate the Vibrio cholerae in seafood sample. The results showed that MPN-real time PCR of the samples from wet market had a maximum of 1100 MPN/g compare to 93 MPN/g enumerated from the MPN plate. The MPN-real time PCR in the samples from supermarket indicated 290 MPN/g as compared to 240 MPN/g enumerated from the MPN plate. The standard curves showed that there was a good linear correlation between the Ct values. The minimum level of detection of Vibrio cholerae standard DNA at targeted gene was 3 x 10-5 ng/l.
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP368 I61 vol. 19(1) Call Number. |
| AGROVOC Term: | Vibrio cholerae |
| AGROVOC Term: | Hygiene |
| AGROVOC Term: | Consumers |
| AGROVOC Term: | Food processing |
| AGROVOC Term: | Waterborne diseases |
| AGROVOC Term: | Human nutrition |
| AGROVOC Term: | Aquaculture |
| AGROVOC Term: | Trematode infections |
| AGROVOC Term: | Pathogenic bacteria |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/22401 |
Actions (login required)
 |
View Item |
