Changes of bioactive components in germinated paddy rice (Oryza sativa L.)

Jirapa K., . and Jarae Y., . and Phanee R., . and Jirasak K., . Changes of bioactive components in germinated paddy rice (Oryza sativa L.). pp. 229-236. ISSN 2231-7546

Effects of soaking conditions and germination conditions on phenolic flavonoid gamma amino butyric acid (GABA) contents and antioxidant activity of five germinated paddy rice cultivars were investigated. The rices were soaked in distilled water pH 7.0 at room temperature (30C) for 24 h followed by germinating in aerobic incubator at 35C for 12 24 36 and 48 hours. In this study the total phenolic and contents were determined by Folin-Ciocalteu reagent and aluminium trichloride respectively germinated rice at 12 h showed the highest levels. 1 1-Diphenyl-2-picryl hydrazyl (DPPH) and 2 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to determine the radical scavenging activity (IC50) germinated rice at 12 h were highest. The phenolic and flavonoid contents including antioxidant activities by DPPH and ABTS of the germinated rice at 12 h were averagely about 2.96 7.93 3.64 and 5.52 times higher than in paddy rice respectively. GABA content increased consistent with germination time. At 24 h of germination the GABA contents were highest which were averagely about 4.12 times higher than in paddy rices. After 24 h of germination phenolic and flavonoid contents and antioxidant activities in all rice cultivars were decreased while GABA concentrations were decreased after 36 h of germination. The results showed high significant correlations between IC50 and phenolic content and between phenolic and flavonoid contents during germination. However GABA content had no correlation with phenolic and flavonoid contents. From the results accumulation and disappearance of these bioactive compounds in rice produced by germination method suggested that germination condition could improve nutrient levels and support effective uses of germinated rice grains for consumption for pharmaceutical application.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/23%20(01)%202016/(34).p...

Abstract

Effects of soaking conditions and germination conditions on phenolic flavonoid gamma amino butyric acid (GABA) contents and antioxidant activity of five germinated paddy rice cultivars were investigated. The rices were soaked in distilled water pH 7.0 at room temperature (30C) for 24 h followed by germinating in aerobic incubator at 35C for 12 24 36 and 48 hours. In this study the total phenolic and contents were determined by Folin-Ciocalteu reagent and aluminium trichloride respectively germinated rice at 12 h showed the highest levels. 1 1-Diphenyl-2-picryl hydrazyl (DPPH) and 2 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to determine the radical scavenging activity (IC50) germinated rice at 12 h were highest. The phenolic and flavonoid contents including antioxidant activities by DPPH and ABTS of the germinated rice at 12 h were averagely about 2.96 7.93 3.64 and 5.52 times higher than in paddy rice respectively. GABA content increased consistent with germination time. At 24 h of germination the GABA contents were highest which were averagely about 4.12 times higher than in paddy rices. After 24 h of germination phenolic and flavonoid contents and antioxidant activities in all rice cultivars were decreased while GABA concentrations were decreased after 36 h of germination. The results showed high significant correlations between IC50 and phenolic content and between phenolic and flavonoid contents during germination. However GABA content had no correlation with phenolic and flavonoid contents. From the results accumulation and disappearance of these bioactive compounds in rice produced by germination method suggested that germination condition could improve nutrient levels and support effective uses of germinated rice grains for consumption for pharmaceutical application.

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Item Type:	Article
AGROVOC Term:	Oryza sativa
AGROVOC Term:	Paddy
AGROVOC Term:	Rice
AGROVOC Term:	Germination
AGROVOC Term:	Soaking
AGROVOC Term:	Flavonoids
AGROVOC Term:	Phenolic content
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22483

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