Molecular detection of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) isolates in raw chicken meat

Zahirul I. K., . and Nurhardy A. D., . and Siti Rokiah A., . and Aklilu E., . and Mokhtar A., . Molecular detection of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) isolates in raw chicken meat. pp. 322-325. ISSN 2231-7546

Multi-drug resistant staphylococci including methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus epidermidis (MRSE) are among the emerging pathogens and have become a threat to both human and animals. Foods of animal origin can easily be contaminated by these bacteria if handled unhygienically or exposed to contaminated environmental surfaces. The objective of this study was to investigate the occurrence of MRSA and MRSE in raw chicken meat sold at wet markets in Kota Bharu Kelantan Malaysia. One hundred fresh raw chicken meat samples were collected from three different wet markets in Kota Bharu Kelantan. Routine isolation and identification selective media (Brilliance MRSA2 agar) antimicrobial sensitivity test (AST) minimum inhibitory concentration test (MIC) and polymerase chain reaction (PCR) amplification of nucA gene and the resistant gene mecA were conducted. Based on bacteriology results and growth on selective media MRSA and MRSE were detected in 43 (43/100) of the raw chicken meat samples. Using the PCR assay 77 (34/43) isolates were positive for nucA gene. The detection of these emerging multi-drug resistant bacteria in chicken meat intended for human consumption implies the potential contamination of food items by the bacteria which in turn may pose risk to the public health.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/23%20(01)%202016/(47).p...

Abstract

Multi-drug resistant staphylococci including methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus epidermidis (MRSE) are among the emerging pathogens and have become a threat to both human and animals. Foods of animal origin can easily be contaminated by these bacteria if handled unhygienically or exposed to contaminated environmental surfaces. The objective of this study was to investigate the occurrence of MRSA and MRSE in raw chicken meat sold at wet markets in Kota Bharu Kelantan Malaysia. One hundred fresh raw chicken meat samples were collected from three different wet markets in Kota Bharu Kelantan. Routine isolation and identification selective media (Brilliance MRSA2 agar) antimicrobial sensitivity test (AST) minimum inhibitory concentration test (MIC) and polymerase chain reaction (PCR) amplification of nucA gene and the resistant gene mecA were conducted. Based on bacteriology results and growth on selective media MRSA and MRSE were detected in 43 (43/100) of the raw chicken meat samples. Using the PCR assay 77 (34/43) isolates were positive for nucA gene. The detection of these emerging multi-drug resistant bacteria in chicken meat intended for human consumption implies the potential contamination of food items by the bacteria which in turn may pose risk to the public health.

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Item Type:	Article
AGROVOC Term:	Staphylococcus aureus
AGROVOC Term:	Staphylococcus epidermidis
AGROVOC Term:	Antimicrobial resistance
AGROVOC Term:	Chicken meat
AGROVOC Term:	Oxacillin
AGROVOC Term:	Pathogens
AGROVOC Term:	Contamination
AGROVOC Term:	Bacteria
AGROVOC Term:	Public health
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22495

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