Detection and antibiotic susceptibility profiles of Listeria monocytogenes in wildlife and water samples in Kubah National Park Sarawak Malaysia

Fazira A. A., . and Velnetti L., . and Samuel L., . and Micky V., . and Lesley M. B., . and Awang A. S. A. H., . and Kasing A., . Detection and antibiotic susceptibility profiles of Listeria monocytogenes in wildlife and water samples in Kubah National Park Sarawak Malaysia. pp. 360-365. ISSN 2231-7546

This study was conducted to detect the presence of Listeria monocytogenes (L. monocytogenes) and screen for its antibiotic susceptibility characteristic from wildlife and water samples at Kubah National Park Sarawak Malaysia. Samples collected were incubated and streaked on selective medium PALCAM agar to confirm the presence of Listeria spp. before they were further tested using molecular analysis. Specific Polymerase Chain Reaction (PCR) assay were performed to target specific virulence gene haemolysin gene hlyA to further distinguish the presence of this pathogenic bacteria in the samples. Overall out of the 30 samples tested 10 samples were confirmed as to contain L. monocytogenes strains and selected to subsequent antibiotic susceptibility test. Susceptibility patterns to 10 antibiotics were investigated among the L. monocytogenes strains. All strains were uniformly resistant to tetracycline and erythromycin. On the other hand all strains were sensitive to gentamycin and tobramycin. The multiple antibiotic resistance shown by the strains in this study indicate the potential health hazard associated with the possible transmission between wildlife and water to its surrounding environment especially visitors and workers of Kubah National Park Sarawak Malaysia.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/23%20(01)%202016/(52).p...

Abstract

This study was conducted to detect the presence of Listeria monocytogenes (L. monocytogenes) and screen for its antibiotic susceptibility characteristic from wildlife and water samples at Kubah National Park Sarawak Malaysia. Samples collected were incubated and streaked on selective medium PALCAM agar to confirm the presence of Listeria spp. before they were further tested using molecular analysis. Specific Polymerase Chain Reaction (PCR) assay were performed to target specific virulence gene haemolysin gene hlyA to further distinguish the presence of this pathogenic bacteria in the samples. Overall out of the 30 samples tested 10 samples were confirmed as to contain L. monocytogenes strains and selected to subsequent antibiotic susceptibility test. Susceptibility patterns to 10 antibiotics were investigated among the L. monocytogenes strains. All strains were uniformly resistant to tetracycline and erythromycin. On the other hand all strains were sensitive to gentamycin and tobramycin. The multiple antibiotic resistance shown by the strains in this study indicate the potential health hazard associated with the possible transmission between wildlife and water to its surrounding environment especially visitors and workers of Kubah National Park Sarawak Malaysia.

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Item Type:	Article
AGROVOC Term:	Listeria monocytogenes
AGROVOC Term:	Antibiotics
AGROVOC Term:	Susceptibility to infection
AGROVOC Term:	Tetracyclines
AGROVOC Term:	Erythromycin
AGROVOC Term:	Gentamycin
AGROVOC Term:	Wildlife
AGROVOC Term:	Pathogenic bacteria
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 16:56
URI:	http://webagris.upm.edu.my/id/eprint/22501

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