Prebiotic fructooligosaccharide production from yeast strain ML1

Kaenpanao P., . and Piwpan P., . and Jaturapiree P., . Prebiotic fructooligosaccharide production from yeast strain ML1. pp. 425-428. ISSN 2231-7546

Fructooligosaccharides (FOS) are non-digestible oligosaccharides mainly composed of 1-kestose (GF2) nystose (GF3) and 1F--fructofuranosyl nystose (GF4) which are recognized as prebiotics because they can stimulate the bifidobacteria growth in the human colon. The aim of this work was to evaluate the factors affecting the production of fructooligosaccharides using sucrose as the substrate by a yeast strain ML1 isolated from sugarcane juice. The effects of sucrose concentrations (50-150 g/L) yeast extract concentrations (10-30 g/L) and agitation speeds (75-300 rpm) were studied in a 3L fermenter. It was found that sucrose concentration was the most effective parameter. This factor showed a positive influence on FOS yields. The optimized conditions for FOS production were 150 g/L sucrose 20 g/L yeast extract with the agitation speed of 150 rpm. Under these controlled conditions the maximum specific growth rate and the FOS concentration were obtained approximately 0.27 h-1 and 65 g/L respectively.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/23%20(01)%202016/(64).p...

Abstract

Fructooligosaccharides (FOS) are non-digestible oligosaccharides mainly composed of 1-kestose (GF2) nystose (GF3) and 1F--fructofuranosyl nystose (GF4) which are recognized as prebiotics because they can stimulate the bifidobacteria growth in the human colon. The aim of this work was to evaluate the factors affecting the production of fructooligosaccharides using sucrose as the substrate by a yeast strain ML1 isolated from sugarcane juice. The effects of sucrose concentrations (50-150 g/L) yeast extract concentrations (10-30 g/L) and agitation speeds (75-300 rpm) were studied in a 3L fermenter. It was found that sucrose concentration was the most effective parameter. This factor showed a positive influence on FOS yields. The optimized conditions for FOS production were 150 g/L sucrose 20 g/L yeast extract with the agitation speed of 150 rpm. Under these controlled conditions the maximum specific growth rate and the FOS concentration were obtained approximately 0.27 h-1 and 65 g/L respectively.

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Item Type:	Article
AGROVOC Term:	Oligosaccharides
AGROVOC Term:	Yeasts
AGROVOC Term:	Extracts
AGROVOC Term:	Sugarcane juice
AGROVOC Term:	Sucrose
AGROVOC Term:	Thailand
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22513

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