Polyphenol fractions of Pithecellobium jiringa cause cell mortality via attenuating matrix metalloproteinase expression in lung and breast cancer cells in vitro

Yanti, . and Hartanto A., . and Wulandari Y. R. E., . and Suhartono M. T., . Polyphenol fractions of Pithecellobium jiringa cause cell mortality via attenuating matrix metalloproteinase expression in lung and breast cancer cells in vitro. pp. 592-598. ISSN 2231-7546

Phytochemical compounds from plants have been used as promising therapeutics to prevent and treat various diseases including cancer. Pithecellobium jiringa seed known as jengkol has possessed pharmacological effects such as antioxidant antimicrobial and anti-inflammatory activities. In this study anticancer activity of polyphenol fractions derived from P. jiringa parts (peel seed coat and seed) was measured by modulating cell mortality and matrix metalloproteinases (MMPs) expression in A549 lung and MCF7 breast cancer cells using the 3-(4 5-dimethyl-2-thiazolyl)-2 5-diphenyl-2H-tetrazolium bromide (MTT) gelatin zymography and Real Time-PCR assays. Polyphenol fraction from each part of P. jiringa was extracted in 70 ethanol and identified using pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS). MTT profiles showed that polyphenol fractions from P. jiringa parts dose dependently increased cell mortality of A549 and MCF7 cancer cells. Gelatin zymographic profiles revealed that P. jiringa polyphenol fractions at lowest dose (5 g mL-1) significantly decreased MMP-2 and MMP-9 activities secreted from A549 cells. At gene level polyphenol fractions exhibited dosage effect on the decreased expression of MMP-2 mRNA in both A549 and MCF7 cancer cells. In summary polyphenol fractions from P. jiringa parts may exert anticancer effect via attenuating MMP-2 expression in vitro.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/23%20(02)%202016/(19).p...

Abstract

Phytochemical compounds from plants have been used as promising therapeutics to prevent and treat various diseases including cancer. Pithecellobium jiringa seed known as jengkol has possessed pharmacological effects such as antioxidant antimicrobial and anti-inflammatory activities. In this study anticancer activity of polyphenol fractions derived from P. jiringa parts (peel seed coat and seed) was measured by modulating cell mortality and matrix metalloproteinases (MMPs) expression in A549 lung and MCF7 breast cancer cells using the 3-(4 5-dimethyl-2-thiazolyl)-2 5-diphenyl-2H-tetrazolium bromide (MTT) gelatin zymography and Real Time-PCR assays. Polyphenol fraction from each part of P. jiringa was extracted in 70 ethanol and identified using pyrolysis-Gas Chromatography/Mass Spectrometry (Py-GC/MS). MTT profiles showed that polyphenol fractions from P. jiringa parts dose dependently increased cell mortality of A549 and MCF7 cancer cells. Gelatin zymographic profiles revealed that P. jiringa polyphenol fractions at lowest dose (5 g mL-1) significantly decreased MMP-2 and MMP-9 activities secreted from A549 cells. At gene level polyphenol fractions exhibited dosage effect on the decreased expression of MMP-2 mRNA in both A549 and MCF7 cancer cells. In summary polyphenol fractions from P. jiringa parts may exert anticancer effect via attenuating MMP-2 expression in vitro.

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Item Type:	Article
AGROVOC Term:	Pithecellobium
AGROVOC Term:	Polyphenols
AGROVOC Term:	Extraction
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Antiinflammatory agents
AGROVOC Term:	Antioxidants
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Polymerase chain reaction
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22544

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