Antimicrobial and cell surface hydrophobicity effects of chemically synthesized fermented foxtail millet meal fraction peptide (FFMp10) mutantson Escherichia coli ATCC 8099 strain

Amadou I., . and Sun G. W., . and Gbadamosi O. S., . and Le G. W., . Antimicrobial and cell surface hydrophobicity effects of chemically synthesized fermented foxtail millet meal fraction peptide (FFMp10) mutantson Escherichia coli ATCC 8099 strain. pp. 708-714. ISSN 2231-7546

This study aimed to verify the effect of chemically synthesized short peptides (LHALLL) previously identified from a fermented foxtail millet meal fraction (FFMp10) on the cell surface hydrophobicity (CSH) biomass growth DNA binding ability of Escherichia coli ATCC 8099. Proteolytic responses of these peptides were also tested using the high performance liquid chromatography (HPLC). The HPLC elution profiles revealed that FFMp10 and its four mutants (Mp1 Mp2 Mp3 and Mp4) peptides responded to 1 h pepsin treatment. However FFMp10 and Mp1 showed reduction in the retention time and Mp2 increased the retention time though Mp3 and Mp4 were more resistant to this proteolytic digestion than FFMp10 and Mp1. Values of CSH were significantly higher (P0.05) with FFMp10 Mp1 and Mp2 2 h treatment on bacteria whereas lowered with Mp3 and Mp4 treatment. Overall peptide treatment which lowered the rate of bacterial biomass growth when compared to control. Slight E. coli ATCC 8099 DNA bindings were observed in lane 2 3 and lane 3 respectively for Mp1 and Mp2 incubation. This suggests that these short peptides reduced hydrophobicity of bacterial but not the viability. Thus provide evidence of effect of these short peptides on the E coli ATCC 8099 growth.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/23%20(02)%202016/(35).p...

Abstract

This study aimed to verify the effect of chemically synthesized short peptides (LHALLL) previously identified from a fermented foxtail millet meal fraction (FFMp10) on the cell surface hydrophobicity (CSH) biomass growth DNA binding ability of Escherichia coli ATCC 8099. Proteolytic responses of these peptides were also tested using the high performance liquid chromatography (HPLC). The HPLC elution profiles revealed that FFMp10 and its four mutants (Mp1 Mp2 Mp3 and Mp4) peptides responded to 1 h pepsin treatment. However FFMp10 and Mp1 showed reduction in the retention time and Mp2 increased the retention time though Mp3 and Mp4 were more resistant to this proteolytic digestion than FFMp10 and Mp1. Values of CSH were significantly higher (P0.05) with FFMp10 Mp1 and Mp2 2 h treatment on bacteria whereas lowered with Mp3 and Mp4 treatment. Overall peptide treatment which lowered the rate of bacterial biomass growth when compared to control. Slight E. coli ATCC 8099 DNA bindings were observed in lane 2 3 and lane 3 respectively for Mp1 and Mp2 incubation. This suggests that these short peptides reduced hydrophobicity of bacterial but not the viability. Thus provide evidence of effect of these short peptides on the E coli ATCC 8099 growth.

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Item Type:	Article
AGROVOC Term:	Escherichia coli
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Hydrophobicity
AGROVOC Term:	Foxtail millet
AGROVOC Term:	Biomass
AGROVOC Term:	DNA
AGROVOC Term:	High performance liquid chromatography
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/22604

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