Citation
Habeeb S., . and Yazaji S., . and Al-Amir L., . Optimization of glucose isomerase production from Streptomyces sp. SH10 using the response surface methodology. pp. 756-761. ISSN 2231-7546
Abstract
The effect of environmental factors on glucose isomerase production by Streptomyces roseiscleroticus was studied using the statistics test Response Surface Methodology (RSM). Results of RSM revealed that the highest production of glucose isomerase reached a maximum level of 13.6 U/ml. The optimum conditions for the production of the enzyme by submerged culture were achieved using broth medium containing 1.5 of xylose as a sole source of carbon and as an inducer for enzyme production in addition to 0.05 magnesium sulfate and 0.05 cobalt chloride as metal ion sources. The initial pH was 6 during an incubation period of 48 hours at 25C. Applying the optimum conditions obtained 13.6 U/ml enzyme activity and indicated that the activity increased approximately 175 in comparison with 7.44 U/ml before applying the optimum conditions.
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Abstract
The effect of environmental factors on glucose isomerase production by Streptomyces roseiscleroticus was studied using the statistics test Response Surface Methodology (RSM). Results of RSM revealed that the highest production of glucose isomerase reached a maximum level of 13.6 U/ml. The optimum conditions for the production of the enzyme by submerged culture were achieved using broth medium containing 1.5 of xylose as a sole source of carbon and as an inducer for enzyme production in addition to 0.05 magnesium sulfate and 0.05 cobalt chloride as metal ion sources. The initial pH was 6 during an incubation period of 48 hours at 25C. Applying the optimum conditions obtained 13.6 U/ml enzyme activity and indicated that the activity increased approximately 175 in comparison with 7.44 U/ml before applying the optimum conditions.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Streptomyces |
| AGROVOC Term: | Phosphoglucose isomerase |
| AGROVOC Term: | Production |
| AGROVOC Term: | Optimization methods |
| AGROVOC Term: | Environmental factors |
| AGROVOC Term: | Enzymes |
| AGROVOC Term: | Xylose |
| AGROVOC Term: | Magnesium |
| AGROVOC Term: | Cobalt |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/22610 |
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