Gender identification of domesticated chicken using a PCR-based method


Citation

Teh Richard Swee Aun, . and Jayaraj Vijaya Kumaran, . Gender identification of domesticated chicken using a PCR-based method. pp. 329-336. ISSN 1511-3701

Abstract

The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD-W and CHD-Z genes in the chickens chromosome Z and chromosome W respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method.


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Abstract

The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD-W and CHD-Z genes in the chickens chromosome Z and chromosome W respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method.

Additional Metadata

[error in script]
Item Type: Article
AGROVOC Term: Chickens
AGROVOC Term: Gender
AGROVOC Term: Identification
AGROVOC Term: PCR
AGROVOC Term: Sex determination (identification)
AGROVOC Term: Sex chromosomes
AGROVOC Term: DNA
AGROVOC Term: Extraction
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/22833

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