Properties of glutamate dehydrogenase in developing legume fruit. I. The effects of chelating agents


Citation

M. Marziah, . Properties of glutamate dehydrogenase in developing legume fruit. I. The effects of chelating agents. pp. 154-159. ISSN 0126-6128

Abstract

A study was conducted to investigate the effects of EDTA and Chelex-100 on the glutamate dehydrogenase activity from a crude extract of the developing seeds of Lupinus angustifolius. The enzyme was not inhibited by Chelex-100; instead it was highly stimulated and its activity when treated with Chelex-100 was higher than that of the control. Glutamate dehydrogenase however was inhibited by 5mM EDTA stimulated at 30mM EDTA and inhibited again at 45mM EDTA.


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Abstract

A study was conducted to investigate the effects of EDTA and Chelex-100 on the glutamate dehydrogenase activity from a crude extract of the developing seeds of Lupinus angustifolius. The enzyme was not inhibited by Chelex-100; instead it was highly stimulated and its activity when treated with Chelex-100 was higher than that of the control. Glutamate dehydrogenase however was inhibited by 5mM EDTA stimulated at 30mM EDTA and inhibited again at 45mM EDTA.

Additional Metadata

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Item Type: Article
AGROVOC Term: Dehydrogenases
AGROVOC Term: Legumes
AGROVOC Term: Chelating agents
AGROVOC Term: Lupinus
AGROVOC Term: Glutamate dehydrogenase
AGROVOC Term: Seeds
AGROVOC Term: Inhibition
AGROVOC Term: Citric acid cycle
AGROVOC Term: Amino acids
AGROVOC Term: Chemical reagents
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 24 Apr 2025 06:27
URI: http://webagris.upm.edu.my/id/eprint/22858

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