Relationship between in vitro cell culture cytotocity and sweat-extractable dithiocarbamates in natural rubber latex gloves.

Abraham E.K., . and Ramesh P., . and Joseph R., . and Kumari T.V., . and Mohanan P.V., . and Remakumari V.M., . Relationship between in vitro cell culture cytotocity and sweat-extractable dithiocarbamates in natural rubber latex gloves. pp. 103-119. ISSN 1511-1768

Cytotoxicity of some of the commercially available natural rubber latex gloves was evaluated by in vitro cell culture test using L929 mouse fibroblast cell lines. The cytotoxic potential ranged from non-cytotoxic to severely cytotoxic for the different brands tested. Since the cytotoxicity of latex products could be related to the release of leachable and toxic residual chemicals the different brands of gloves were extracted in a physiologically simulated medium namely artificial sweat. The results revealed that a rubber accelerator zinc diethyldithiocarbamate (ZDEC) was present in the gloves as residues and released into the artificial sweat. The amount of ZDEC released into the artificial sweat (sweat-extractable ZDEC) was estimated using high performance liquid chromatography. It was observed that there existed a linear relationship between the amount of sweat-extractable ZDEC and the cytotoxic potential of gloves represented in grades ranging from 0 to 3 as per ISO 10993-5 (1999) standard. This preliminary investigation showed that the amount of sweat-extractable ZDEC is a good indicator of the cytotoxicity potential of gloves and hence the artificial sweat extraction may be further developed as a reliable test method to screen new latex medical products/formulations.

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Abstract

Cytotoxicity of some of the commercially available natural rubber latex gloves was evaluated by in vitro cell culture test using L929 mouse fibroblast cell lines. The cytotoxic potential ranged from non-cytotoxic to severely cytotoxic for the different brands tested. Since the cytotoxicity of latex products could be related to the release of leachable and toxic residual chemicals the different brands of gloves were extracted in a physiologically simulated medium namely artificial sweat. The results revealed that a rubber accelerator zinc diethyldithiocarbamate (ZDEC) was present in the gloves as residues and released into the artificial sweat. The amount of ZDEC released into the artificial sweat (sweat-extractable ZDEC) was estimated using high performance liquid chromatography. It was observed that there existed a linear relationship between the amount of sweat-extractable ZDEC and the cytotoxic potential of gloves represented in grades ranging from 0 to 3 as per ISO 10993-5 (1999) standard. This preliminary investigation showed that the amount of sweat-extractable ZDEC is a good indicator of the cytotoxicity potential of gloves and hence the artificial sweat extraction may be further developed as a reliable test method to screen new latex medical products/formulations.

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Item Type:	Article
AGROVOC Term:	Cell culture
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Sweat
AGROVOC Term:	Dithiocarbamates
AGROVOC Term:	Natural rubber
AGROVOC Term:	Latex
AGROVOC Term:	In vitro culture
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/22923

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