Citation
Yang K. L., . and Norazreen Abd Rahman, . and Ong M. T., . and Sunderasan E., . and Lam K. L., . Cell viability assay guided fractionation of natural rubber latex sera. pp. 195-202. ISSN 1511-1768
Abstract
Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionation of latex B- and C-serum using cell viability assay (anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B- and C-serum against distilled water caused some of its constituents to precipitate then a brief centrifugation separated their fractions “ dialysed B-serum precipitate (DBP) dialysed B-serum supernatant (DBS) dialysed C-serum precipitate (DCP) and dialysed C-serum supernatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B- and C-serum precipitate from their supernatant. Cell viability assay performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B- and C-serum fractions. Chromatographic separation of DBP DCP and DCS followed by cell viability assay will shed light if sub-fractions with further improved anti-proliferative activity are attainable.
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Abstract
Natural rubber latex sera have been investigated for anti-proliferative property on cancer cell lines. The present report describes fractionation of latex B- and C-serum using cell viability assay (anti-proliferative activity) as a guide to narrow down on the active constituents. Dialysis of B- and C-serum against distilled water caused some of its constituents to precipitate then a brief centrifugation separated their fractions “ dialysed B-serum precipitate (DBP) dialysed B-serum supernatant (DBS) dialysed C-serum precipitate (DCP) and dialysed C-serum supernatant (DCS). Boiling of B- and C-serum resulted in extensive precipitation; a brief centrifugation separated the boiled B- and C-serum precipitate from their supernatant. Cell viability assay performed on two human cancer-origin cell lines revealed a marked increase in anti-proliferative activity in the dialysed C-serum fractions and in DBP but diminished in DBS as well as in the boiled B- and C-serum fractions. Chromatographic separation of DBP DCP and DCS followed by cell viability assay will shed light if sub-fractions with further improved anti-proliferative activity are attainable.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Latex |
| AGROVOC Term: | Rubber |
| AGROVOC Term: | Natural rubber |
| AGROVOC Term: | Tissue proliferation |
| AGROVOC Term: | Cancer (disease) |
| AGROVOC Term: | Viability |
| AGROVOC Term: | Dialysis |
| AGROVOC Term: | Distilled water |
| AGROVOC Term: | Precipitation |
| AGROVOC Term: | Centrifugation |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 28 Apr 2025 02:30 |
| URI: | http://webagris.upm.edu.my/id/eprint/22981 |
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