cDNA cloning and expression analysis of three components of SCFCOI1 complex in Hevea brasiliensis

Wang L-F., . and Ma R-F., . and Peng S-Q., . and Tian W-M., . cDNA cloning and expression analysis of three components of SCFCOI1 complex in Hevea brasiliensis. pp. 141-152. ISSN 1511-1768

To gain a deeper understanding of jasmonate signaling in specific tissues three of full-length cDNAs referred to as HbCUL1 HbSKP1 and HbRBX1 were isolated from laticifers of rubber tree by the rapid amplification of cDNA ends (RACE) method and analysed by sequence analysis and real-time RT-PCR. The putative proteins HbCUL1 HbSKP1 and HbRBX1 had specific domains of CULLIN superfamily and Cullin Nedd8 Skp1-POZ and Skp1 and RING superfamily respectively. HbCUL1 and HbSKP1 were most related to AtCUL1 and AtASK1 among the members of Cullins and ASKs from Arabidopsis respectively. Methyl jasmonate was more effective than ethylene on up-regulating the expression of HbCUL1 HbSKP1 and HbRBX1. These data suggest that HbCUL1 HbSKP1 and HbRBX1 may be the invariant components of SCFCOI1 complex of jasmonate signaling in laticifers of rubber tree.

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Abstract

To gain a deeper understanding of jasmonate signaling in specific tissues three of full-length cDNAs referred to as HbCUL1 HbSKP1 and HbRBX1 were isolated from laticifers of rubber tree by the rapid amplification of cDNA ends (RACE) method and analysed by sequence analysis and real-time RT-PCR. The putative proteins HbCUL1 HbSKP1 and HbRBX1 had specific domains of CULLIN superfamily and Cullin Nedd8 Skp1-POZ and Skp1 and RING superfamily respectively. HbCUL1 and HbSKP1 were most related to AtCUL1 and AtASK1 among the members of Cullins and ASKs from Arabidopsis respectively. Methyl jasmonate was more effective than ethylene on up-regulating the expression of HbCUL1 HbSKP1 and HbRBX1. These data suggest that HbCUL1 HbSKP1 and HbRBX1 may be the invariant components of SCFCOI1 complex of jasmonate signaling in laticifers of rubber tree.

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Item Type:	Article
AGROVOC Term:	Cloning
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Jasmonates
AGROVOC Term:	Meja (methyl jasmonate)
AGROVOC Term:	Ethylene
AGROVOC Term:	Natural rubber
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/22994

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