Citation
Huang T.D., . and Ziyun W., . and Huang H.S., . and Hua Y.W., . Establishment and characterisation of Hevea cell suspension cultures. pp. 45-57. ISSN 1511-1768
Abstract
A simple and rapid protocol for establishment of Hevea suspension cultures is presented. H. brasiliensis H. nitida suspension cultures with uniformly dispersed cells and cell aggregates were obtained after one to two months of culture. The growth kinetics study showed a typical sigmoidal profile including lag phase log phase or exponential phase linear phase and deceleration phase while the doubling time was 25 days. Sedimented cell volume (SCV) fresh weight (FW) growth index (GI) of FW the decrease of electrical conductivity (EC) in the medium had a very good linear correlation with dry weight (DW) or GI of DW. The optimum maintenance medium for Hevea suspension cultures was the modified MS medium supplemented with 0.2 mg/L 2 4-D 2 mg/L NAA 1 mg/L kinetin 50 g/L sucrose 0.2 g/L L-Asparagine and 0.4 g/L casein hydrolysate and coconut water 5 (v/v) in which suspension cultures were still well grown with multiplication after incubating in optimised suspension medium (OSM) for more than one year. This cell suspension cultures would be used for protoplast isolation large-scale clonal propagation preservation of genetic resources genetic transformation and establishment of bioreactors in Hevea.
Download File
Full text available from:
Official URL: http://vitaldoc.lgm.gov.my:8060/vital/access/servi...
|
Abstract
A simple and rapid protocol for establishment of Hevea suspension cultures is presented. H. brasiliensis H. nitida suspension cultures with uniformly dispersed cells and cell aggregates were obtained after one to two months of culture. The growth kinetics study showed a typical sigmoidal profile including lag phase log phase or exponential phase linear phase and deceleration phase while the doubling time was 25 days. Sedimented cell volume (SCV) fresh weight (FW) growth index (GI) of FW the decrease of electrical conductivity (EC) in the medium had a very good linear correlation with dry weight (DW) or GI of DW. The optimum maintenance medium for Hevea suspension cultures was the modified MS medium supplemented with 0.2 mg/L 2 4-D 2 mg/L NAA 1 mg/L kinetin 50 g/L sucrose 0.2 g/L L-Asparagine and 0.4 g/L casein hydrolysate and coconut water 5 (v/v) in which suspension cultures were still well grown with multiplication after incubating in optimised suspension medium (OSM) for more than one year. This cell suspension cultures would be used for protoplast isolation large-scale clonal propagation preservation of genetic resources genetic transformation and establishment of bioreactors in Hevea.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Natural rubber |
| AGROVOC Term: | Hevea brasiliensis |
| AGROVOC Term: | Biomass |
| AGROVOC Term: | Cells |
| AGROVOC Term: | Plant growth regulators |
| AGROVOC Term: | Yields |
| AGROVOC Term: | Suspension culture |
| AGROVOC Term: | Protoplasts |
| AGROVOC Term: | Genetic transformation |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23043 |
Actions (login required)
 |
View Item |
