NRESTdb: Access to the transcriptome of natural rubber latex

Chee C. H., . and Ahmad Fuad Hilmi M., . and Chow K. S., . and Mohd Noor M. I., . and Mohd Yunus S., . NRESTdb: Access to the transcriptome of natural rubber latex. pp. 229-238. ISSN 1511-1768

Current technological advancement of DNA sequencing has afforded opportunities to analyse genes on a larger scale. In natural rubber research DNA sequencing technology has been used in the generation of approximately 10 000 DNA sequences representing genes expressed in the latex of Hevea brasiliensis (Muell. Arg.) clone RRIM600. These sequence data more popularly known as expressed sequence tags (ESTs) can be analysed using bioinformatics tools to facilitate gene discovery and provide information regarding the putative function and expression profile of latex genes. We have developed NRESTdb a Natural Rubber EST database to provide easy access and rapid analysis of these data. To our knowledge this is the first publicly available EST database for H. brasiliensis. In addition to serving as a repository for individual latex EST and clustered sequences this database allows users to view mine and retrieve useful information regarding: the transcriptome of natural rubber latex including matches with existing domains and gene sequences available in public databases; description based on the Gene Ontology nomenclature; homologues identified via comparative sequence analysis; and enhanced data sets of special interest namely rubber biosynthesis and latex allergy.

Full text available from: Official URL: http://vitaldoc.lgm.gov.my:8060/vital/access/servi...

Abstract

Current technological advancement of DNA sequencing has afforded opportunities to analyse genes on a larger scale. In natural rubber research DNA sequencing technology has been used in the generation of approximately 10 000 DNA sequences representing genes expressed in the latex of Hevea brasiliensis (Muell. Arg.) clone RRIM600. These sequence data more popularly known as expressed sequence tags (ESTs) can be analysed using bioinformatics tools to facilitate gene discovery and provide information regarding the putative function and expression profile of latex genes. We have developed NRESTdb a Natural Rubber EST database to provide easy access and rapid analysis of these data. To our knowledge this is the first publicly available EST database for H. brasiliensis. In addition to serving as a repository for individual latex EST and clustered sequences this database allows users to view mine and retrieve useful information regarding: the transcriptome of natural rubber latex including matches with existing domains and gene sequences available in public databases; description based on the Gene Ontology nomenclature; homologues identified via comparative sequence analysis; and enhanced data sets of special interest namely rubber biosynthesis and latex allergy.

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Item Type:	Article
AGROVOC Term:	Natural rubber
AGROVOC Term:	Bioinformatics
AGROVOC Term:	Databases
AGROVOC Term:	genomics
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Allergens
AGROVOC Term:	DNA sequence
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:10
URI:	http://webagris.upm.edu.my/id/eprint/23055

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