Yield stimulation of the rubber tree (RRIM 600) with ethephon does not increase latex Hev b 6 protein or mRNA.

Arokiaraj P., . and Yeang H.Y., . Yield stimulation of the rubber tree (RRIM 600) with ethephon does not increase latex Hev b 6 protein or mRNA. pp. 61-71. ISSN 1511-1768

Hevea brasiliensis latex hevein is an allergenic protein designated the allergen name Hev b 6 by the International Union of Immunological Societies. A study was undertaken to determine if latex flow stimulation by ethephon increased Hev b 6 concentration in the latex from mature rubber trees. A two-site immunoenzymatic assay with antibodies specific for hevein revealed no significant differences in Hev b 6 content in control trees and Hevea trees stimulated with ethephon. The mRNA transcript levels of hevein determined by northern analysis appeared similar for control and stimulated trees. Using the relative ratio method for Real Time PCR assessment the steady-state transcript levels of hevein were also similar for control and stimulated trees. It was concluded that latex from mature Hevea trees stimulated with ethephon did not result in an increase in hevein mRNA transcripts or in the translated protein. There was therefore no evidence from this study that ethephon stimulation increased allergenicity of latex by increasing Hev b 6 content.

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Abstract

Hevea brasiliensis latex hevein is an allergenic protein designated the allergen name Hev b 6 by the International Union of Immunological Societies. A study was undertaken to determine if latex flow stimulation by ethephon increased Hev b 6 concentration in the latex from mature rubber trees. A two-site immunoenzymatic assay with antibodies specific for hevein revealed no significant differences in Hev b 6 content in control trees and Hevea trees stimulated with ethephon. The mRNA transcript levels of hevein determined by northern analysis appeared similar for control and stimulated trees. Using the relative ratio method for Real Time PCR assessment the steady-state transcript levels of hevein were also similar for control and stimulated trees. It was concluded that latex from mature Hevea trees stimulated with ethephon did not result in an increase in hevein mRNA transcripts or in the translated protein. There was therefore no evidence from this study that ethephon stimulation increased allergenicity of latex by increasing Hev b 6 content.

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Item Type:	Article
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Allergens
AGROVOC Term:	Ethephon
AGROVOC Term:	Yields
AGROVOC Term:	Stimulation
AGROVOC Term:	Proteins
AGROVOC Term:	Pathogenesis
AGROVOC Term:	Latex
AGROVOC Term:	PCR
AGROVOC Term:	Rubber crops
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:11
URI:	http://webagris.upm.edu.my/id/eprint/23089

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