The factor contributing to higher extractable protein content in natural rubber latex glove as determined by ASTM D5712-99 over ASTM D5712-95 and its relation to allergen content


Citation

Hasma H., . and Dazylah D., . and Siti Nor Qamarina M., . The factor contributing to higher extractable protein content in natural rubber latex glove as determined by ASTM D5712-99 over ASTM D5712-95 and its relation to allergen content. pp. 73-77. ISSN 1511-1768

Abstract

Values for glove extractable protein content determined by ASTM D5712-99 were generally higher than corresponding values obtained by ASTM D5712-95. This resulted from the different procedures used in the two assays. Among all the variables investigated it was ascertained that differences in extraction medium were the main contributing factor. Extraction with 25 mM phosphate-buffered saline as in ASTM D5712-99 gave higher protein content than extraction with water as in ASTM D5712-95. The same was true of the allergen values of the extracts.


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Abstract

Values for glove extractable protein content determined by ASTM D5712-99 were generally higher than corresponding values obtained by ASTM D5712-95. This resulted from the different procedures used in the two assays. Among all the variables investigated it was ascertained that differences in extraction medium were the main contributing factor. Extraction with 25 mM phosphate-buffered saline as in ASTM D5712-99 gave higher protein content than extraction with water as in ASTM D5712-95. The same was true of the allergen values of the extracts.

Additional Metadata

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Item Type: Article
AGROVOC Term: Proteins
AGROVOC Term: Natural rubber
AGROVOC Term: Allergens
AGROVOC Term: Extraction
AGROVOC Term: Binding proteins
AGROVOC Term: Latex
AGROVOC Term: Protein content
AGROVOC Term: Rubber industry
Geographical Term: Malaysia
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 28 Apr 2025 03:11
URI: http://webagris.upm.edu.my/id/eprint/23095

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