An assessment of stimulation of Hevea brasiliensis rubber biosynthesis by eIF-5A protein-enriched bacteria lysates

Chow K. S., . and Latifah A., . and Salimah M. L., . and Faridah Y., . An assessment of stimulation of Hevea brasiliensis rubber biosynthesis by eIF-5A protein-enriched bacteria lysates. pp. 251-259. ISSN 1511-1768

The effect of six unique isoforms of Hevea brasiliensis eukaryotic translation initiation factor 5A (eIF-5A) previously reported as a rubber biosynthesis stimulator protein (RBSP) was evaluated using biosynthesis assays containing zone 2 rubber particles and radio labelled isopentenyl diphosphate (14C-IPP). Recombinant RBSP proteins were over-expressed as N-terminal glutathione-S-transferase (GST)-tagged proteins in a bacterial system. Crude extracts of six RBSP-GST isoforms were added to rubber biosynthesis assays in increasing volumes. Results showed that increases in the amount of each RBSP-GST isoform corresponded with increases in the level of 14C-IPP incorporation above that of the control which contained no recombinant protein. Statistical data analysis revealed that three RBSP isoforms increased 14C-IPP incorporation significantly compared to the GST control where the RBSP isoform with the highest stimulatory effect produced a 45 increase in IPP incorporation compared to GST.

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Abstract

The effect of six unique isoforms of Hevea brasiliensis eukaryotic translation initiation factor 5A (eIF-5A) previously reported as a rubber biosynthesis stimulator protein (RBSP) was evaluated using biosynthesis assays containing zone 2 rubber particles and radio labelled isopentenyl diphosphate (14C-IPP). Recombinant RBSP proteins were over-expressed as N-terminal glutathione-S-transferase (GST)-tagged proteins in a bacterial system. Crude extracts of six RBSP-GST isoforms were added to rubber biosynthesis assays in increasing volumes. Results showed that increases in the amount of each RBSP-GST isoform corresponded with increases in the level of 14C-IPP incorporation above that of the control which contained no recombinant protein. Statistical data analysis revealed that three RBSP isoforms increased 14C-IPP incorporation significantly compared to the GST control where the RBSP isoform with the highest stimulatory effect produced a 45 increase in IPP incorporation compared to GST.

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Item Type:	Article
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Biosynthesis
AGROVOC Term:	Bacterial proteins
AGROVOC Term:	Glutathione
AGROVOC Term:	Rubber industry
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:11
URI:	http://webagris.upm.edu.my/id/eprint/23103

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