Citation
Siti Arija M. A., . and Lau C. H., . and Chan J. L., . and Samsidar H., . and Yeang H. Y., . and Hamilton R. G., . and Loke Y. H., . Hev b 1 Hev b 2 and Hev b 3 contents in natural rubber latex and powdered latex gloves. pp. 167-178. ISSN 1511-1768
Abstract
The contents of three latex allergens Hev b 1 Hev b 2 and Hev b 3 were compared in 24 latex examination gloves and in fresh natural rubber latex using immunoassays that employed a two-site ELISA format. Hev b 1 Hev b 2 and Hev b 3 that were extractable from the powdered latex gloves were found to represent only a very small proportion of their contents in fresh latex and a very small proportion of the total extractable glove proteins. Of the three allergens studied only Hev b 2 content was significantly correlated with total protein content. Hence total protein content did not always reflect the contents of the individual latex allergens. Of the three allergens studied Hev b 2 and Hev b 3 contents were significantly correlated with binding to IgE pooled from latex-allergic patients. One brand of glove had undetectable levels of the three individual latex allergens indicating that it was feasible to remove a large proportion of the allergens during glove manufacture.
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Abstract
The contents of three latex allergens Hev b 1 Hev b 2 and Hev b 3 were compared in 24 latex examination gloves and in fresh natural rubber latex using immunoassays that employed a two-site ELISA format. Hev b 1 Hev b 2 and Hev b 3 that were extractable from the powdered latex gloves were found to represent only a very small proportion of their contents in fresh latex and a very small proportion of the total extractable glove proteins. Of the three allergens studied only Hev b 2 content was significantly correlated with total protein content. Hence total protein content did not always reflect the contents of the individual latex allergens. Of the three allergens studied Hev b 2 and Hev b 3 contents were significantly correlated with binding to IgE pooled from latex-allergic patients. One brand of glove had undetectable levels of the three individual latex allergens indicating that it was feasible to remove a large proportion of the allergens during glove manufacture.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Natural rubber |
| AGROVOC Term: | Latex |
| AGROVOC Term: | Allergens |
| AGROVOC Term: | Protein content |
| AGROVOC Term: | ELISA |
| AGROVOC Term: | Particle size |
| AGROVOC Term: | Sodium |
| AGROVOC Term: | Centrifugation |
| AGROVOC Term: | Bovines |
| AGROVOC Term: | Inhibition |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/23176 |
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