Isolation and characterisation of latex cyanogenic glucosidase in Hevea brasiliensis

Ward M. A., . and Sunderasan E., . and Yeang H. Y., . Isolation and characterisation of latex cyanogenic glucosidase in Hevea brasiliensis. pp. 244-252. ISSN 1511-1768

Cyanogenic glucosidases play an important role in defence against pathogen attack in plants. Hevea brasiliensis latex glucosidase was isolated from the latex B-serum by dialysis-induced protein precipitation and gel filtration. Follwing SDS-polyacrylamide gel electrophoresis carried out under reducing conditions the protein was observed to be about 57 kDa. Partial amino acid sequences for the protein matched published sequences of several plant glucosidases including those of cynogenic glucosidases. Enzyme assays revealed that the protein possessed glucosidase activity this being confirmed by isozyme staining of the protein after electrophoretic separation on native polyacrylamide gels. Enzyme activity was also observed when linamarin was used as the specific substrate confirming that the protein was a cyanogenic glucosidase (linamarase) as suggested by the partial amino acid sequences. The latex cyanogenic glucosidase is a component of an allergic protein complex Hev b 4. Cloning and sequencing of the complete cDNA encoding the glucosidase is ongoing.

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Abstract

Cyanogenic glucosidases play an important role in defence against pathogen attack in plants. Hevea brasiliensis latex glucosidase was isolated from the latex B-serum by dialysis-induced protein precipitation and gel filtration. Follwing SDS-polyacrylamide gel electrophoresis carried out under reducing conditions the protein was observed to be about 57 kDa. Partial amino acid sequences for the protein matched published sequences of several plant glucosidases including those of cynogenic glucosidases. Enzyme assays revealed that the protein possessed glucosidase activity this being confirmed by isozyme staining of the protein after electrophoretic separation on native polyacrylamide gels. Enzyme activity was also observed when linamarin was used as the specific substrate confirming that the protein was a cyanogenic glucosidase (linamarase) as suggested by the partial amino acid sequences. The latex cyanogenic glucosidase is a component of an allergic protein complex Hev b 4. Cloning and sequencing of the complete cDNA encoding the glucosidase is ongoing.

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Item Type:	Article
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Isolation
AGROVOC Term:	Latex
AGROVOC Term:	Pathogens
AGROVOC Term:	Linamarase
AGROVOC Term:	Amino acid sequences
AGROVOC Term:	Allergens
AGROVOC Term:	Glucosidases
AGROVOC Term:	Identification
AGROVOC Term:	Sediment
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23183

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