Citation
Hirel B., . and Prevot J. C., . and Jacob J. L., . and Clement A., . and Pujade-Renaud V., . Glutamine synthetase of Hevea brasiliensis latex: characterisation and physiological regulation. pp. 56-68. ISSN 1511-1768
Abstract
Regular exploitation of rubber tree causes intense metabolic activity in latex cells located in the phloem associated with the regeneration of the latex cellular components removed by tapping. In this context glutamine synthetase (GS) plays a prominent role by ensuring ammonium fixation necessary for the regeneration of latex nitrogenous components. in latex CS activity was detected in the cytosol only. The enzyme was purified and its main physico-chemical characteristics were determined. Its molecular mass was 450 kDa for the native form and 45 kDa 5 kDa for the sub-unit. The pH optimum was found to be 8.0 and variations in the physiological pH range (6.4 to 7.2) strongly affected GS activity. Km for ammonium glutamate and ATP were estimated at 8 uM 2 uM 4 mM 0.5 mM and 0.6 mM 0.05 mM respectively. Comparison with the cytosol physiological parameters showed the importance of ATP concentration for GS activity regulation. Functioning of the purified enzyme in a paraphysiological (ultrafiltrated protein-free) medium as compared with an optimised control medium demonstrated that physiological ATP concentrations were significantly limiting for GS potential activity. Treatment with ethephon increases latex production both by increasing latex flow duration and by stimulating the regeneration of metabolism. In this paper we demonstrated that ethephon treatment induced a concomitant increase in pH ATP concentration and GS activity. These results suggest that biochemical mechanisms involving pH and ATP participate in the regulation of GS activity in response to ethylene in addition to the previously observed stimulation of gene expression.
Download File
Full text available from:
Official URL: http://vitaldoc.lgm.gov.my:8060/vital/access/servi...
|
Abstract
Regular exploitation of rubber tree causes intense metabolic activity in latex cells located in the phloem associated with the regeneration of the latex cellular components removed by tapping. In this context glutamine synthetase (GS) plays a prominent role by ensuring ammonium fixation necessary for the regeneration of latex nitrogenous components. in latex CS activity was detected in the cytosol only. The enzyme was purified and its main physico-chemical characteristics were determined. Its molecular mass was 450 kDa for the native form and 45 kDa 5 kDa for the sub-unit. The pH optimum was found to be 8.0 and variations in the physiological pH range (6.4 to 7.2) strongly affected GS activity. Km for ammonium glutamate and ATP were estimated at 8 uM 2 uM 4 mM 0.5 mM and 0.6 mM 0.05 mM respectively. Comparison with the cytosol physiological parameters showed the importance of ATP concentration for GS activity regulation. Functioning of the purified enzyme in a paraphysiological (ultrafiltrated protein-free) medium as compared with an optimised control medium demonstrated that physiological ATP concentrations were significantly limiting for GS potential activity. Treatment with ethephon increases latex production both by increasing latex flow duration and by stimulating the regeneration of metabolism. In this paper we demonstrated that ethephon treatment induced a concomitant increase in pH ATP concentration and GS activity. These results suggest that biochemical mechanisms involving pH and ATP participate in the regulation of GS activity in response to ethylene in addition to the previously observed stimulation of gene expression.
Additional Metadata
Item Type: | Article |
---|---|
AGROVOC Term: | Hevea brasiliensis |
AGROVOC Term: | Latex |
AGROVOC Term: | Glutamine synthetase |
AGROVOC Term: | Physiological regulation |
AGROVOC Term: | Exploitation of resources |
AGROVOC Term: | Tapping |
AGROVOC Term: | Ethephon |
AGROVOC Term: | Metabolism |
AGROVOC Term: | Biochemical reactions |
AGROVOC Term: | Cytoplasm |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:28 |
URI: | http://webagris.upm.edu.my/id/eprint/23188 |
Actions (login required)
![]() |
View Item |