Protein marker for tapping panel dryness identified as the small rubber particle protein (Hev b 3 )

Chan J. L., . and Xiao X., . and Yeang H. Y., . and Samsidar H., . and Luo S., . Protein marker for tapping panel dryness identified as the small rubber particle protein (Hev b 3 ). pp. 42-52. ISSN 1511-1768

Previous studies undertaken by different researchers using electrophoretic techniques have identified in Hevea latex several putative protein markers that are linked to tapping panel dryness (TPD). A major marker that was frequently encountered was a latex protein of 22 kDa - 26 kDa. In the present study analysis by Western blot and two-site ELISA showed this marker to be identical to the 22.4 kDa small rubber particle protein (SRPP) which is an allergenic peptide also known as Hev b 3. The SRPP level in C-serum of partially dry trees showed a significant increase over that of the normal yielding trees confirming that the marker was SRPP. However no similar increase was found in the SRPP levels when assayed in the whole latex. These results suggested that there was no de novo synthesis of SRPP with the onset of partial dryness and the protein is not therefore a direct marker for incipient tree dryness. The apparent increase of the protein observed in the C-serum was likely to be due to increased numbers of small rubber particles failing to separate out of the C-serum upon centrifugation. SRPP may nevertheless be indirectly associated with TPD by its being an indicator of latex stability.

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Abstract

Previous studies undertaken by different researchers using electrophoretic techniques have identified in Hevea latex several putative protein markers that are linked to tapping panel dryness (TPD). A major marker that was frequently encountered was a latex protein of 22 kDa - 26 kDa. In the present study analysis by Western blot and two-site ELISA showed this marker to be identical to the 22.4 kDa small rubber particle protein (SRPP) which is an allergenic peptide also known as Hev b 3. The SRPP level in C-serum of partially dry trees showed a significant increase over that of the normal yielding trees confirming that the marker was SRPP. However no similar increase was found in the SRPP levels when assayed in the whole latex. These results suggested that there was no de novo synthesis of SRPP with the onset of partial dryness and the protein is not therefore a direct marker for incipient tree dryness. The apparent increase of the protein observed in the C-serum was likely to be due to increased numbers of small rubber particles failing to separate out of the C-serum upon centrifugation. SRPP may nevertheless be indirectly associated with TPD by its being an indicator of latex stability.

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Item Type:	Article
AGROVOC Term:	Proteins
AGROVOC Term:	Rubber crops
AGROVOC Term:	Particle size
AGROVOC Term:	Tapping
AGROVOC Term:	Latex
AGROVOC Term:	Peptides
AGROVOC Term:	Protein synthesis
AGROVOC Term:	Hevea brasiliensis
AGROVOC Term:	Molecular markers
AGROVOC Term:	Immunological tests
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23208

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