Allergenicity of latex gloves with reference to latex protein sensitive individuals in a Canadian population

Yip Esah, . and Sussman Gordon L., . Allergenicity of latex gloves with reference to latex protein sensitive individuals in a Canadian population. pp. 129-141. ISSN 1511-1768

Allergenicity of latex gloves with varying levels of total extractable proteins was studied by evaluating their allergic responses elicited if any in latex sensitive individuals of Canadian origin. Total extractable protein (EP) content of 30 latex medical gloves (powdered and powder-free) was determined by the modified Lowry method according to both the Rubber Research Institute of Malaysia (RRIM) and the American Society for Testing and Materials (ASTM) protocols. While EPRRIM values ranged from 865 mg/g to 10 mg/g EPASTM values varied from 870 mg/g to 30 mg/g. A total of 30 latex allergic volunteers were skin-prick tested with the glove extracts. Results showed that gloves with high extractable protein contents always tend to elicit positive allergic reactions. On the other hand much reduced or no allergic responses were associated with gloves having low extractable proteins regardless of whether the gloves were powdered or powder-free. More than 60 of negative allergic responses were consistently observed at EPRRIM of about 100 mg/g and lower and at EPASTM of less than 50 mg/g. The total extractable protein content of latex gloves was found to be significantly correlated to their allergenicity notwithstanding that the protein measurements were not fully allergen specific. Correlation coefficients r of 0.94 for EPRRIM and 0.88 for EPASTM were obtained. This finding confirms an earlier report that showed close correlation between allergenicity and EPRRIM in a study based on a Finnish population of different genetic make-up.

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Abstract

Allergenicity of latex gloves with varying levels of total extractable proteins was studied by evaluating their allergic responses elicited if any in latex sensitive individuals of Canadian origin. Total extractable protein (EP) content of 30 latex medical gloves (powdered and powder-free) was determined by the modified Lowry method according to both the Rubber Research Institute of Malaysia (RRIM) and the American Society for Testing and Materials (ASTM) protocols. While EPRRIM values ranged from 865 mg/g to 10 mg/g EPASTM values varied from 870 mg/g to 30 mg/g. A total of 30 latex allergic volunteers were skin-prick tested with the glove extracts. Results showed that gloves with high extractable protein contents always tend to elicit positive allergic reactions. On the other hand much reduced or no allergic responses were associated with gloves having low extractable proteins regardless of whether the gloves were powdered or powder-free. More than 60 of negative allergic responses were consistently observed at EPRRIM of about 100 mg/g and lower and at EPASTM of less than 50 mg/g. The total extractable protein content of latex gloves was found to be significantly correlated to their allergenicity notwithstanding that the protein measurements were not fully allergen specific. Correlation coefficients r of 0.94 for EPRRIM and 0.88 for EPASTM were obtained. This finding confirms an earlier report that showed close correlation between allergenicity and EPRRIM in a study based on a Finnish population of different genetic make-up.

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Item Type:	Article
AGROVOC Term:	Latex
AGROVOC Term:	Skins
AGROVOC Term:	Allergic reactions
AGROVOC Term:	Powders
AGROVOC Term:	Protein content
AGROVOC Term:	Allergens
AGROVOC Term:	Hypersensitivity
AGROVOC Term:	Protein concentrates
AGROVOC Term:	Precipitation
AGROVOC Term:	Trichloroacetic acid
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	28 Apr 2025 03:24
URI:	http://webagris.upm.edu.my/id/eprint/23214

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