In vitro interactions of rubber chemical additives with cells from varying lineages

Mok K. L., . and Evans S. W., . and Evan s S. D., . In vitro interactions of rubber chemical additives with cells from varying lineages. pp. 200-213. ISSN 1511-1768

In cytotoxicity study it is possible that in vitro cell responses will vary depending on the cell lineage. This consequently affects the toxicity ranking and the threshold levels considered safe for use. This study aims to examine the probable variations in the cytotoxic effects elicited by rubber chemical additives evaluated against three different cell lines U937 (monocytic) HeLa (epithelial) and L929 (fibroblastic) cells. Additives tested were zinc diethyldithiocarbamate (ZDEC) zinc dibutyldithiocarbamate (ZBUD) zinc-2-mercaptobenzothiazole (ZMBT) zinc oxide (ZnO) and 2 2-methylene-bis(4-methyl-6-tert-butylphenol) (2246). Cell response was quantified using the 3-4 5-dimethylthiazol-2-yl-2 5-diphenyltetrazolium bromide (MTT) assay which measures the cell metabolic activity via the reduction of MTT salt by dehydrogenase enzymes in living cells. Results show that ZDEC was least tolerated by all three cell lines followed by ZBUD while the toxicity ranking of ZMBT ZnO and 2246 changes depending on the types of cell. The lethal chemical concentrations (LC50) against the three cell lines were constant for ZDEC and ZMBT but varied for ZBUD ZnO and 2246. This indicates cell-dependent toxicity among some of the rubber chemical additives. Caution should therefore be exercised when using these toxicity threshold values to gauge the safety levels for use.

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Abstract

In cytotoxicity study it is possible that in vitro cell responses will vary depending on the cell lineage. This consequently affects the toxicity ranking and the threshold levels considered safe for use. This study aims to examine the probable variations in the cytotoxic effects elicited by rubber chemical additives evaluated against three different cell lines U937 (monocytic) HeLa (epithelial) and L929 (fibroblastic) cells. Additives tested were zinc diethyldithiocarbamate (ZDEC) zinc dibutyldithiocarbamate (ZBUD) zinc-2-mercaptobenzothiazole (ZMBT) zinc oxide (ZnO) and 2 2-methylene-bis(4-methyl-6-tert-butylphenol) (2246). Cell response was quantified using the 3-4 5-dimethylthiazol-2-yl-2 5-diphenyltetrazolium bromide (MTT) assay which measures the cell metabolic activity via the reduction of MTT salt by dehydrogenase enzymes in living cells. Results show that ZDEC was least tolerated by all three cell lines followed by ZBUD while the toxicity ranking of ZMBT ZnO and 2246 changes depending on the types of cell. The lethal chemical concentrations (LC50) against the three cell lines were constant for ZDEC and ZMBT but varied for ZBUD ZnO and 2246. This indicates cell-dependent toxicity among some of the rubber chemical additives. Caution should therefore be exercised when using these toxicity threshold values to gauge the safety levels for use.

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Item Type:	Article
AGROVOC Term:	Additives
AGROVOC Term:	In vitro
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Toxicity
AGROVOC Term:	Rubber
AGROVOC Term:	Dehydrogenases
AGROVOC Term:	Chemical concentration
AGROVOC Term:	Latex
AGROVOC Term:	Aneuploids
AGROVOC Term:	Carcinoma
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23221

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