Phase partitioning for enzyme separation: an overview and recent applications

Ketnawa Sunantha, . and Rungraeng N., . and Rawdkuen Saroat, . Phase partitioning for enzyme separation: an overview and recent applications. pp. 1-24. ISSN ISSN(Online):22317546

Conventional purification techniques those were used to recover and purify enzymes are composed of several steps. Besides procedures have proven laborious costly time consuming and yielding low recovery. Hence an alternative recovery technique such as an aqueous two phase system (ATPS) thermoseparation (TMP) and three phase partitioning (TPP) is created to solve these disadvantages. Multiple factors affecting recovery and purity are investigated including the polymer molecular weight and concentration the salt type and concentration the system pH the NaCl addition and the total number of cycles. The alternative recovery technique has been widely applied for separating and purifying enzymes from various sources such as plants (bromelain papain invertase polyphenol oxidase and trypsin inhibitors) animals (trypsin -chymotrypsin chymosin pepsin and luciferase) and microbial (lipase -amylase -galactosidase and cyclodextrin glycosyltransferase). In this article the backgrounds and phase forming materials are presented. The parameters that affect the enzymes partitioning behavior as well as recent applications of these methods are reviewed and summarized.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/24%20(01)%202017/(1).pd...

Abstract

Conventional purification techniques those were used to recover and purify enzymes are composed of several steps. Besides procedures have proven laborious costly time consuming and yielding low recovery. Hence an alternative recovery technique such as an aqueous two phase system (ATPS) thermoseparation (TMP) and three phase partitioning (TPP) is created to solve these disadvantages. Multiple factors affecting recovery and purity are investigated including the polymer molecular weight and concentration the salt type and concentration the system pH the NaCl addition and the total number of cycles. The alternative recovery technique has been widely applied for separating and purifying enzymes from various sources such as plants (bromelain papain invertase polyphenol oxidase and trypsin inhibitors) animals (trypsin -chymotrypsin chymosin pepsin and luciferase) and microbial (lipase -amylase -galactosidase and cyclodextrin glycosyltransferase). In this article the backgrounds and phase forming materials are presented. The parameters that affect the enzymes partitioning behavior as well as recent applications of these methods are reviewed and summarized.

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Item Type:	Article
AGROVOC Term:	Enzymes
AGROVOC Term:	Polymers
AGROVOC Term:	Molecular weight
AGROVOC Term:	Purification
AGROVOC Term:	Separation
AGROVOC Term:	Plants
AGROVOC Term:	Papain
AGROVOC Term:	Invertase
AGROVOC Term:	Polyphenol oxidase
AGROVOC Term:	Trypsin inhibitors
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:28
URI:	http://webagris.upm.edu.my/id/eprint/23246

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